Isolation of coated vesicles, plain synaptic vesicles, and flocculent material from a crude synaptosome fraction of guinea pig whole brain.
Isolation of coated vesicles, plain synaptic vesicles, and flocculent material from a crude synaptosome fraction of guinea pig whole brain.
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DOI：
10.1083/jcb.58.1.135
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发表时间：
1973-07
期刊：
影响因子：
--
通讯作者：
Kadota T
中科院分区：
文献类型：
--
作者：
Kadota K;Kadota T
通过差速离心和硫酸铵盐析，从粗突触体中制备了两种囊泡组分和一种非囊泡组分。组分1含有包被囊泡的混合物，该物质被认为是来自于外套（壳碎片）的分解，以及普通的突触囊泡。组分2含有普通突触囊泡和絮状物质的混合物。级分3仅含有絮状物质。通过Sephadex柱部分纯化级分1和3。馏分3不含壳碎片，但含有更精细的絮凝材料，它被认为是由单位颗粒单独出现或连接在一起成链状或无定形聚集体。每个单位粒子似乎有四个亚基，在这里被称为四体。四体有时似乎附着在普通突触囊泡的表面。此外，四体的聚集体也可能构成突触前致密投射结构的一部分。
Two vesicular fractions and one nonvesicular fraction were prepared from crude synaptosomes by differential centrifugation and salting out with ammonium sulfate. Fraction 1 contained a mixture of coated vesicles, material thought to be derived from breakdown of the coats (shell fragments), and plain synaptic vesicles. Fraction 2 contained a mixture of plain synaptic vesicles and flocculent material. Fraction 3 contained flocculent material only. Fractions 1 and 3 were partially purified by passage through a Sephadex column. Fraction 3 contained no shell fragments but contained finer flocculent material which, it is suggested, is composed of unit particles either occurring singly or linked together into chainlike or amorphous aggregates. Each unit particle appears to have four subunits and is here referred to as a tetrasome. Tetrasomes sometimes appear to be attached to the surfaces of the plain synaptic vesicles. Also, it is possible that aggregates of tetrasomes form part of the structure of the presynaptic dense projections.