Pro-inflammatory cytokine IL1 β 1 participates in promoting parturition related pathways in the ovoviviparous teleost black rockfish (Sebastes schlegelii)
Pro-inflammatory cytokine IL1 β 1 participates in promoting parturition related pathways in the ovoviviparous teleost black rockfish (Sebastes schlegelii)
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DOI：
10.1093/biolre/ioad100
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发表时间：
2023-08
影响因子：
3.6
通讯作者：
Shaojing Yan;Likang Lyu;Xiaojie Wang;H. Wen;Yun Li;Jianshuang Li;Yijia Yao;Chenpeng Zuo;Songyang Xie;Zhijun Wang;Xin Qi
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文献类型：
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作者：
Shaojing Yan;Likang Lyu;Xiaojie Wang;H. Wen;Yun Li;Jianshuang Li;Yijia Yao;Chenpeng Zuo;Songyang Xie;Zhijun Wang;Xin Qi
摘要在进化过程中，为了最大限度地提高后代的存活率，进化出了卵生、胎生和卵生三种生殖策略。在哺乳动物中，开始分娩的关键是母胎界面的炎症反应。白细胞介素1β (interleukin 1β, IL1β)是一种促炎性细胞因子，在人的分娩过程中起着重要作用。虽然我们对il - 1β1在硬骨鱼分娩过程中的作用知之甚少，但我们将il - 1β1在硬骨鱼（一种卵生硬骨鱼，在怀孕期间通过类似胎盘的结构为5万多个胚胎提供超过60%的营养供应）诱导分娩中的作用作为研究模型。本研究在基因克隆的基础上，通过原位杂交检测了Il1b1及其受体在围产期的表达模式，以及在卵巢中的定位。分析了原核表达系统获得的重组il - 1β1 （rIL1β1）处理围产期卵巢原代细胞转录组学数据中的不同表达基因。差异表达基因、功能富集和通路分析主要包括免疫应答、信号转导和细胞死亡。总之，我们的研究为il - 1β1在卵生硬骨鱼分娩中的潜在作用提供了新的见解。il - β1通过促进黑岩鱼PG的合成，改变围产期卵巢从静止状态到炎症激活状态，从而导致分娩。
Abstract Along with the evolution process, the reproductive strategies evolved including oviparity, viviparity and ovoviviparity, to fit the residential environment maximize the survival rate of the off spring. In mammals, the key to the initiation of parturition is the inflammatory response at the maternal-fetal interface. As a pro-inflammatory cytokine, interleukin 1 beta (IL1β) plays an important role in the process of human parturition. While less is known about IL1β1 in teleost parturition, identification of the functions of IL1β1 in inducing the parturition, black rockfish, an ovoviviparity teleost, which provides over 60% nutrition supply for over 50 000 embryos though a placenta like structure during pregnant, was employed as the research model. In the present study, based on the gene cloning, we detected the expression pattern of both Il1b1 and its receptor perinatal period, as well as the localization to the ovary by in situ hybridization. The different expression genes in transcriptomic data of perinatal primary ovarian cells treated with the recombinant IL1β1 (rIL1β1) obtained by prokaryotic expression system were analyzed. Differentially expressed genes, functional enrichment and pathway analysis mainly included immune response, signal transduction and cell death. In summary, our research provides novel insights into the potential role of IL1β1 in the parturition of ovoviviparity teleost. Summary Sentence Ilβ1 alters the perinatal ovary from quiescent status to inflammatory activation status leading to parturition by promoting PG synthesis in black rockfish.