Nicotine sensitization increases dendritic length and spine density in the nucleus accumbens and cingulate cortex
Nicotine sensitization increases dendritic length and spine density in the nucleus accumbens and cingulate cortex
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DOI：
10.1016/s0006-8993(01)02201-6
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发表时间：
2001-04-27
期刊：
影响因子：
2.9
通讯作者：
Kolb, B
中科院分区：
文献类型：
--
作者：
Brown, RW;Kolb, B
本研究探讨了重复给予尼古丁（0.7 mg/kg）对丘脑核（NAcc）、前额叶皮层（Cg 3）和顶叶皮层（Par 1）中树突形态的影响。使动物习惯于运动箱3天，习惯后，在皮下注射尼古丁或盐水后立即将大鼠置于运动室中，每隔一天持续5周。大鼠在每次尼古丁注射后表现出对初始活动减退反应的耐受性，随后在每次注射后活动增加（行为致敏）。在戒烟2周后的尼古丁激发试验中，这种活性的增加仍然存在。尼古丁激发日后一周，对所有大鼠进行灌注并取出大脑。我们使用Golgi-Cox程序对这些大脑进行染色，并使用照相机清晰程序分析来自脑桥核（N Ace）、内侧额叶皮质（Cg 3）和顶叶皮质（Par 1）的树突。结果显示，接受尼古丁的大鼠在NAcc和Cg 3脑区的树突长度和棘密度相对于对照组有所增加，但Par 1没有增加。在NAcc中观察到的增加明显大于安非他明或可卡因，并讨论了可能的潜在机制。(C)2001 Elsevier Science B. V.保留所有权利。
This study investigated the effects of repeated administrations of nicotine (0.7 mg/kg) on dendritic morphology in the nucleus accumbens (NAcc), prefrontal cortex (Cg 3), and parietal cortex (Par 1). Animals were habituated for 3 days to a locomotor box, and after habituation, every second day for 5 weeks rats were placed into the locomotor chamber immediately after a subcutaneous injection of nicotine or saline. Rats demonstrated tolerance to an initial hypoactive response after each nicotine injection, and this was followed by an increase in activity after each injection (behavioral sensitization). This increase in activity was still present on a nicotine challenge after a 2-week abstinence period. One week after the nicotine challenge day, all rats were perfused and brains were removed. These brains we stained using Golgi-Cox procedures, and dendrites from the nucleus accumbens (N Ace), medial frontal cortex (Cg 3) and parietal cortex (Par 1) were analyzed using the camera lucida procedure. Results showed that rats receiving nicotine demonstrated an increase in dendritic length and spine density relative to controls in the NAcc and Cg3 brain areas, but not Par 1. The increase observed in the NAcc was significantly greater than what has been found with amphetamine or cocaine, and possible underlying mechanisms were discussed. (C) 2001 Elsevier Science B.V. All rights reserved.