Epigallocatechin-3-gallate attenuates Porphyromonas gingivalis lipopolysaccharide-enhanced matrix metalloproteinase-1 production through inhibition of interleukin-6 in gingival fibroblasts.
Epigallocatechin-3-gallate attenuates Porphyromonas gingivalis lipopolysaccharide-enhanced matrix metalloproteinase-1 production through inhibition of interleukin-6 in gingival fibroblasts.
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DOI：
10.1902/jop.2013.120714
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发表时间：
2014-05
影响因子：
4.3
通讯作者：
Wan-Chun Wen;Po-Jan Kuo;Cheng-Yang Chiang;Yu-Tang Chin;Martin M. Fu;E. Fu
中科院分区：
文献类型：
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作者：
Wan-Chun Wen;Po-Jan Kuo;Cheng-Yang Chiang;Yu-Tang Chin;Martin M. Fu;E. Fu
背景技术最近的研究表明，绿茶提取物的主要成分表没食子儿茶素-3-没食子酸酯(EGCG)对牙周炎症具有抗炎和抗氧化作用。本体外研究检测了 EGCG 对牙龈卟啉单胞菌 (Pg) 脂多糖 (LPS) 增强的白介素 (IL)-6 和基质金属蛋白酶 (MMP)-1 表达以及核因子 -κ B (NF-κB) 激活的影响。此外，使用人牙龈成纤维细胞 (HGF) 评估了 IL-6 对 LPS 增强的 MMP-1 产生的作用。方法 HGFs是从人牙龈标本中原代培养的。通过细胞活力测试来测试EGCG和LPS的细胞毒性。逆转录聚合酶链反应检测细胞IL-6 mRNA表达量，酶联免疫吸附法检测MMP-1和IL-6蛋白表达量。通过免疫细胞化学和共聚焦激光扫描显微镜评估 NF-κB 的胞质表达和核转位。结果 Pg LPS 显着增加 HGF 中 MMP-1 的产生，而添加 EGCG 则显着减弱这种增强的 MMP-1 产生。 LPS 处理还增加了 IL-6 的 mRNA 和蛋白表达，并刺激 HGF 中 NF-κB 的激活。然而，添加 EGCG 显着减弱了 IL-6 表达和 NF-κB 激活。补充添加 IL-6 显着增强细胞 MMP-1 的产生，而抗 IL-6 抗体则抑制 LPS 增强的 MMP-1 产生。结论 EGCG 可以减弱 HGF 中 Pg LPS 增强的 MMP-1 产生，而这种减弱可能是由于 EGCG 抑制 IL-6 所致。
BACKGROUND Recent studies have shown that epigallocatechin-3-gallate (EGCG), a major constituent of green tea extract, exhibits effects of anti-inflammation and antioxidation on periodontal inflammation. The present in vitro study examines the effect of EGCG on Porphyromonas gingivalis (Pg) lipopolysaccharide (LPS)-enhanced expression of interleukin (IL)-6 and matrix metalloproteinase (MMP)-1, as well as the activation of nuclear factor-kappa B (NF-κB). Furthermore, the role of IL-6 on LPS-enhanced MMP-1 production is evaluated using human gingival fibroblasts (HGFs). METHODS HGFs were primary cultured from human gingiva specimens. The cytotoxicities of EGCG and LPS were tested by cell viability tests. The cellular mRNA expression of IL-6 was determined by reverse-transcription polymerase chain reaction, and the protein expression of MMP-1 and IL-6 was examined by enzyme-linked immunosorbent assay. The cytosol expression and nuclear translocation of NF-κB was evaluated by immunocytochemistry followed by confocal laser scanning microscopy. RESULTS Pg LPS significantly increased MMP-1 production in HGFs, whereas adding EGCG significantly attenuated this enhanced production of MMP-1. LPS treatment also increased the mRNA and protein expression of IL-6 and stimulated NF-κB activation in HGFs. However, the addition of EGCG significantly attenuated the IL-6 expression and NF-κB activation. Supplemental addition of IL-6 significantly enhanced cellular MMP-1 production, whereas anti-IL-6 antibody inhibited LPS-enhanced MMP-1 production. CONCLUSION EGCG could attenuate Pg LPS-enhanced production of MMP-1 in HGFs, whereas this attenuation might be due to the inhibition of IL-6 by EGCG.