Fluctuations and synchrony of RNA synthesis in nucleoli
Fluctuations and synchrony of RNA synthesis in nucleoli
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DOI：
10.1039/c5ib00008d
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发表时间：
2015-01-01
影响因子：
2.5
通讯作者：
Prasad, Paras N.
中科院分区：
文献类型：
--
作者：
Pliss, Artem;Kuzmin, Andrey N.;Prasad, Paras N.
核糖体RNA(RRNA)序列以极高的速率合成，并与核糖体蛋白(r-蛋白质)一起用作组装核糖体前颗粒的构件。虽然人们普遍认为rRNA和r-蛋白的严格调控和协调对于维持细胞内环境的稳定是至关重要的，但对单个细胞中核糖体成分合成的实时动力学仍知之甚少。在这篇通讯中，我们介绍了一种无标记的微波拉曼光谱方法，用于监测活培养细胞中rRNA的合成。这项技术揭示了rRNA产生率的显著快速波动。值得注意的是，位于同一细胞的不同核仁中的核糖体基因的rRNA产量的变化是同步的。我们的发现呼吁开发新的概念来阐明核糖体成分生产的协调。在这方面，数值模拟进一步证明，无论rRNA合成的波动如何，rRNA和r-蛋白质的产生都可以协调。总体而言，我们的定量数据揭示了RNA合成的固有随机速率和基因表达的协调之间的壮观相互作用。
Ribosomal RNA (rRNA) sequences are synthesized at exceptionally high rates and, together with ribosomal proteins (r-proteins), are utilized as building blocks for the assembly of pre-ribosomal particles. Although it is widely acknowledged that tight regulation and coordination of rRNA and r-protein production are fundamentally important for the maintenance of cellular homeostasis, still little is known about the real-time kinetics of the ribosome component synthesis in individual cells. In this communication we introduce a label-free MicroRaman spectrometric approach for monitoring rRNA synthesis in live cultured cells. Remarkably high and rapid fluctuations of rRNA production rates were revealed by this technique. Strikingly, the changes in the rRNA output were synchronous for ribosomal genes located in separate nucleoli of the same cell. Our findings call for the development of new concepts to elucidate the coordination of ribosomal components production. In this regard, numerical modeling further demonstrated that the production of rRNA and r-proteins can be coordinated, regardless of the fluctuations in rRNA synthesis. Overall, our quantitative data reveal a spectacular interplay of inherently stochastic rates of RNA synthesis and the coordination of gene expression.