Substrate stress relaxation regulates cell spreading.
Substrate stress relaxation regulates cell spreading.
复制标题
DOI：
10.1038/ncomms7365
复制
发表时间：
2015-02-19
影响因子：
16.6
通讯作者：
Mooney DJ
中科院分区：
文献类型：
--
作者：
Chaudhuri O;Gu L;Darnell M;Klumpers D;Bencherif SA;Weaver JC;Huebsch N;Mooney DJ
细胞机械转导的研究已经集中在细胞通过测量它们施加在ECM上的牵引力的阻力来感知细胞外基质（ECM）弹性的想法上。然而，这些研究通常利用纯弹性材料作为基底，而生理ECM是粘弹性的，并且表现出应力松弛，使得由细胞施加的细胞牵引力重塑ECM。在这里，我们调查ECM应力松弛对细胞行为的影响，通过计算建模和细胞实验。令人惊讶的是，我们的计算模型和实验都发现，在表现出应力松弛的软基底上培养的细胞的铺展大于在相同模量的弹性基底上铺展的细胞，但与在更硬的弹性基底上铺展的细胞的铺展相似。这些发现挑战了目前关于细胞如何感知和响应ECM的观点。
Studies of cellular mechanotransduction have converged upon the idea that cells sense extracellular matrix (ECM) elasticity by gauging resistance to the traction forces they exert on the ECM. However, these studies typically utilize purely elastic materials as substrates, whereas physiological ECM are viscoelastic, and exhibit stress relaxation, so that cellular traction forces exerted by cells remodel the ECM. Here we investigate the influence of ECM stress relaxation on cell behavior through computational modeling and cellular experiments. Surprisingly, both our computational model and experiments find that spreading for cells cultured on soft substrates that exhibit stress relaxation is greater than cells spreading on elastic substrates of the same modulus, but similar to that of cells spreading on stiffer elastic substrates. These findings challenge the current view of how cells sense and respond to the ECM.