Establishment of a murine, lipopolysaccharide-induced sepsis model for testing anaerobic exercise thresholds and early mobilization
Establishment of a murine, lipopolysaccharide-induced sepsis model for testing anaerobic exercise thresholds and early mobilization
复制标题
建立小鼠脂多糖诱导的脓毒症模型，用于测试无氧运动阈值和早期活动
DOI：
10.1016/j.medidd.2020.100074
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发表时间：
2021
影响因子：
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通讯作者：
Inoue Yoshiaki
中科院分区：
文献类型：
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作者：
Matsuishi Yujiro;Shimojo Nobutake;Hoshino Haruhiko;Enomoto Yuki;Mathis Bryan J.;Oh Sechang;Kawano Satoru;Myoenzono Kanae;Maeda Seiji;Shoda Junichi;Inoue Shigeaki;Inoue Yoshiaki
背景目前已有多种方法预防脓毒症后重症监护综合征，包括早期动员，但其机制和效果仍存在争议。因此，本研究的目的是建立一个脓毒症小鼠模型和分类运动强度在脓毒症的急性期产生一个小鼠脓毒症康复model.MethodsAdult，雄性C57/B6小鼠接受脂多糖（LPS）注射（20 mg/kg）。我们记录了存活率和代谢变化，如静息能量消耗（REE），与对照组相比，LPS给药后72小时。我们还通过代谢监测跑步机观察生命体征（直肠温度和体重变化）、炎症（TNF-α和IL-6）和运动强度。结果LPS诱导后72 h，约30%的小鼠存活，22-24 h REE最低（22 h REE：2.26 ± 0.34 kcal/d，p <0.05）。TNF-α的血液浓度在LPS诱导后12 h时间点最高（41.23 ± 24.39 pg/mL，p <0.05 vs.基线），IL-6在LPS诱导后24 h最高（1476.5 ± 274.7 pg/mL，p <0.05 vs.基线）。与V斜率，我们观察了AT在每个时间点（6小时：14米/分钟，12小时：9米/分钟，24小时：4米/分钟，36小时：10米/分钟，48小时：16米/分钟，60小时：20米/分钟，72小时：22米/分钟），并将这些分为非常低强度运动训练，低强度运动训练，或中等强度运动训练在每个timepoint.ConclusionWe分类运动强度到72小时后，脓毒症恢复使用小鼠模型。这些数据可以作为临床研究的桥梁，以填补脓毒症康复最佳实践的空白。
BackgroundSeveral methods have been proposed to prevent post intensive care syndrome after sepsis, including early mobilization, but controversy remains in mechanisms and outcomes. Therefore, the aim of this study is to establish a septic mouse model and classify exercise intensities during the acute phase of sepsis to generate a murine sepsis rehabilitation model.MethodsAdult, male C57/B6 mice received lipopolysaccharide (LPS) injections (20 mg/kg). We recorded survival rates and metabolic changes, such as resting energy expenditure (REE), compared with controls up to 72 h after LPS administration. We also observed vital signs (rectal temperature and weight change), inflammation (TNF-α and IL-6), and exercise intensity via metabolic monitoring treadmill. We analyzed the anaerobic threshold (AT) by the V-Slope method.ResultAround 30% of the mice survived 72 h after LPS induction with the lowest REE at around 22–24 h later (REE at 22 h: 2.26 ± 0.34 kcal/dayp< 0.05 vs. baseline). Blood concentrations of TNF-α were highest at the 12 h timepoint (41.23 ± 24.39 pg/mL,p< 0.05 vs. baseline) and IL-6 was highest at 24 h after LPS induction (1476.5 ± 274.7 pg/mL,p< 0.05 vs. baseline). With V-Slope, we observed the AT at each timepoint (6 h:14 m/min,12 h: 9 m/min, 24 h:4 m/min,36 h:10 m/min, 48 h:16 m/min, 60 h:20 m/min, 72 h:22 m/min) and classified these into very low intensity exercise training, low intensity exercise training, or moderate intensity exercise training at each timepoint.ConclusionWe classified exercise intensities out to 72 h after sepsis recovery using a mouse model. These data may serve as a bridge to clinical studies to fill gaps in best practice for sepsis rehabilitation.