Induction and biological properties of defective interfering particles of rabies virus
Induction and biological properties of defective interfering particles of rabies virus
复制标题
狂犬病病毒缺陷干扰颗粒的诱导及生物学特性
作者：
T. Wiktor;B. Dietzschold;R. Leamnson;H. Koprowski
描述了一种获得大量符合Huang和Baltimore（1970）所描述的所有标准的缺陷干扰（DI）狂犬病毒颗粒的方法。纯化的狂犬病DI病毒粒子比完整的病毒粒子（180纳米）短得多（60至80纳米），病毒基因组的大小约为正常狂犬病RNA的一半，但具有标准病毒粒子的所有结构蛋白。狂犬病DI病毒粒子对培养细胞和动物均无感染性。通过体外和体内技术确定，对标准病毒复制的干扰是狂犬病毒所特有的。讨论了狂犬病DI病毒粒子在狂犬病病毒感染的致病性和狂犬病活疫苗减毒株的建立中可能发挥的作用。
A method for obtaining large quantities of defective interfering (DI) rabies virus particles that fulfill all the criteria delineated by Huang and Baltimore (1970) is described. The purified rabies DI virion was found to be much shorter (60 to 80 nm) than the complete virion (180 nm) and to have a viral genome of about half the size of normal rabies RNA but with all of the structural proteins of standard virions. Rabies DI virions were noninfectious for both cells in culture and for animals. As determined by in vitro and in vivo techniques, interference with the replication of standard virus was specific to rabies virus. The possible role of rabies DI virion in the pathogenicity of rabies virus infection and in the establishment of attenuated strains for use as live rabies vaccines is discussed.