Increased number of cells and metabolic activity in male human preimplantation embryos following in vitro fertilization.
Increased number of cells and metabolic activity in male human preimplantation embryos following in vitro fertilization.
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DOI：
10.1530/jrf.0.1040165
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发表时间：
1995-05
期刊：
影响因子：
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通讯作者：
P. Ray;J. Conaghan;R. Winston;A. Handyside
中科院分区：
文献类型：
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作者：
P. Ray;J. Conaghan;R. Winston;A. Handyside
检查男性和女性人类植入前胚胎的细胞数量和代谢活动，以确定体外受精（IVF）后男性胚胎是否比女性胚胎更先进。正常受精胚胎的代谢活性每天通过非侵入性测量受精后第2天至第6天期间丙酮酸和葡萄糖的摄取以及乳酸的产生来评估。第6天，通过差异标记和荧光显微镜计数囊胚滋养外胚层和内细胞团的细胞核数量。然后回收细胞核，并使用嵌套引物分别扩增 X 和 Y 染色体上的牙釉蛋白基因和假基因序列来鉴定胚胎的性别。然后回顾性比较男性和女性胚胎的发育。从发育至囊胚阶段的 178 个胚胎中的 69 个（39%）中，确定了 57 个胚胎的性别； 21 名 (37%) 为男性，36 名 (63%) 为女性。雄性胚胎中的细胞数量在第 2 天显着增加（P < 0.005），并且这种差异一直维持到囊胚阶段（滋养外胚层和内细胞团），尽管差异并不总是显着。第 2 天至第 5 天期间，雄性胚胎对丙酮酸的吸收显着较高（P < 0.05）。第 4-5 天，雄性胚胎的葡萄糖摄取和乳酸产生显着较高（P < 0.05）；这种差异在第 5-6 天不显着。根据细胞数量差异推断，与雄性胚胎相比，雌性胚胎从第 2 天开始发育延迟约 4.5 小时。（摘要截断为 250 字）
The number of cells and metabolic activity of male and female human preimplantation embryos were examined to determine whether male embryos are more advanced than female embryos following in vitro fertilization (IVF). The metabolic activity of embryos fertilized normally was assessed daily by non-invasive measurement of pyruvate and glucose uptake and lactate production between days 2 and 6 after insemination. On day 6, the numbers of nuclei from the trophectoderm and inner cell mass of blastocysts were counted by differential labelling and fluorescence microscopy. Nuclei were then recovered and the sex of the embryos identified using nested primers to amplify the amelogenin gene and pseudogene sequences on the X and Y chromosomes, respectively. Development of male and female embryos were then compared retrospectively. From 69 of 178 (39%) embryos that developed to the blastocyst stage, the sex of 57 was determined; 21 (37%) were male and 36 (63%) female. The number of cells in male embryos was significantly greater on day 2 (P < 0.005), and this difference was maintained up to the blastocyst stage (in both the trophectoderm and the inner cell mass), although differences were not always significant. Pyruvate uptake was significantly higher by male embryos between days 2 and 5 (P < 0.05). Glucose uptake and lactate production were significantly higher in male embryos on days 4-5 (P < 0.05); this difference was not significant on days 5-6. Extrapolation from differences in the number of cells indicates that female embryos are approximately 4.5 h delayed in their development from day 2 onwards compared with male embryos.(ABSTRACT TRUNCATED AT 250 WORDS)