Molecular phylogeny of the Gasterosteidae: the importance of using multiple genes
Molecular phylogeny of the Gasterosteidae: the importance of using multiple genes
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DOI：
10.1016/s1055-7903(03)00190-8
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发表时间：
2004-02-01
影响因子：
4.1
通讯作者：
Mattern, MY
中科院分区：
文献类型：
--
作者：
Mattern, MY
腹鱼科是进化生物学中一个重要的模式系统。系统发育关系以前已经建立在形态和行为数据的基础上，但迄今为止还没有人研究过这些关系使用分子特征。本文报道了利用5个线粒体基因（12 S rRNA、16 S rRNA、细胞色素B、ATP酶6和控制区）的序列分析结果。2879 bp的系统发育分析产生了一个最简约的树的一致性指数为72.6%。这棵树与行为+形态学拓扑一致，但有一个例外：Apeltes被列为Pungitius + Culaea的姐妹群，而不是（Pungitius + Culaea）+ Gasterosteus的姐妹群。这项研究强调了在系统发育分析中使用多个线粒体基因的重要性。单独地，这五个基因产生了四个显著不同的拓扑结构，并且如果只有一两个基因被测序的话，可能会给出不同版本的Gasterosteid关系。因此，比较生物学家必须只选择包含多个线粒体基因的树作为研究进化模式和过程的基础。(C)2003 Elsevier Science（美国）。All rights reserved.
The Gasterosteidae is an important model system in evolutionary biology. Phylogenetic relationships have previously been constructed based upon morphological and behavioral data, but to date no one has investigated those relationships using molecular characters. This paper reports the results of an analysis using sequences from five mitochondrial genes (12S rRNA, 16S rRNA, cytochrome b, ATPase 6, and control region). Phylogenetic analysis of 2879 bp produced a single most parsimonious tree with a consistency index of 72.6%. That tree agrees with the behavior + morphology topology, with one exception: Apeltes is placed as the sister group to Pungitius + Culaea, rather than as the sister-group of (Pungitius + Culaea) + Gasterosteus. This study highlights the importance of using multiple mitochondrial genes in a phylogenetic analysis. Separately, the five genes produced four significantly different topologies, and might have given different versions of gasterosteid relationships had only one or two genes been sequenced. It is thus imperative that comparative biologists choose only trees that contain multiple mitochondrial genes as the basis for studies of evolutionary patterns and processes. (C) 2003 Elsevier Science (USA). All rights reserved.