CXCR4 receptor positive spheroid forming cells are responsible for tumor invasion in vitro
CXCR4 receptor positive spheroid forming cells are responsible for tumor invasion in vitro
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DOI：
10.1016/j.canlet.2009.02.005
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发表时间：
2009-07-18
期刊：
影响因子：
9.7
通讯作者：
Alt, Eckhard
中科院分区：
文献类型：
--
作者：
Krohn, Alexander;Song, Yao-Hua;Alt, Eckhard
干细胞已被发现参与乳腺癌的生长，但癌症干细胞在肿瘤生物学（包括转移）中的具体贡献仍不确定。我们发现小鼠乳腺癌细胞系4T1、4T07、167Farn和67NR含有通过CXCR4表达和它们在悬浮培养中形成球状体的能力定义的癌症干细胞。重要的是，我们发现CXCR4表达对于肿瘤侵袭是必不可少的，因为CXCR4中和抗体和CXCR4受体的shRNA敲低都显著降低了肿瘤细胞侵袭。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
Stem cells have been found to be involved in breast cancer growth, but the specific contribution of cancer stem cells in tumor biology, including metastasis, is still uncertain. We found that murine breast cancer cell lines 4T1, 4TO7, 167Farn and 67NR contains cancer stem cells defined by CXCR4 expression and their capability of forming spheroids in suspension culture. Importantly, we showed that CXCR4 expression is essential for tumor invasiveness because both CXCR4 neutralizing antibody and shRNA knockdown of the CXCR4 receptor significantly reduced tumor cell invasion. (C) 2009 Elsevier Ireland Ltd. All rights reserved.