In vivo periodontal tissue regeneration by periodontal ligament stem cells and endothelial cells in three-dimensional cell sheet constructs
In vivo periodontal tissue regeneration by periodontal ligament stem cells and endothelial cells in three-dimensional cell sheet constructs
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DOI：
10.1111/jre.12405
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发表时间：
2017-06-01
影响因子：
3.5
通讯作者：
Zhang, C.
中科院分区：
文献类型：
--
作者：
Panduwawala, C. P.;Zhan, X.;Zhang, C.
慢性牙周炎对牙齿支持组织造成损害，导致成年人牙齿脱落。最近，基于细胞片的方法已被研究，以克服牙周再生的传统细胞治疗程序的局限性。本研究的目的是探讨牙周膜干细胞（PDLSCs）和人脐静脉内皮细胞（HUVECs）的再生潜力在三维（3D）细胞片构建牙周regenerationinvivo.Material和MethodsPDLSCs，HUVECs或两种细胞的共培养物接种到温度响应的培养皿中，并制作完整的细胞片。细胞片包裹在三个不同的组合和皮下植入到免疫缺陷mice.ResultsHistological评价显示，2，4和8周的植入后，牙周韧带样组织的安排，观察周围的实验组相比，与对照组的植入根。在含HUVEC组的牙周区室中也观察到血管腔。骨膜蛋白和骨唾液蛋白免疫染色显示，实验组在第4周和第8周的骨膜韧带再生、牙骨质形成和骨形成明显。在移植的细胞片中的人类细胞通过免疫组织化学染色的存在human mitochondrial.ConclusionsThe三维细胞片为基础的方法可能是潜在的有益的，因此鼓励未来的再生牙周治疗。
Background and ObjectiveChronic periodontitis causes damage to tooth-supporting tissues, resulting in tooth loss in adults. Recently, cell-sheet-based approaches have been studied to overcome the limitations of conventional cytotherapeutic procedures for periodontal regeneration. The purpose of the present study was to investigate the regenerative potential of periodontal ligament stem cells (PDLSCs) and human umbilical vein endothelial cells (HUVECs) in three-dimensional (3D) cell sheet constructs for periodontal regeneration in vivo.Material and MethodsPDLSCs, HUVECs or co-cultures of both cells were seeded onto temperature-responsive culture dishes, and intact cell sheets were fabricated. Cell sheets were wrapped around the prepared human roots in three different combinations and implanted subcutaneously into immunodeficient mice.ResultsHistological evaluation revealed that after 2, 4 and 8 wk of implantation, periodontal ligament-like tissue arrangements were observed around the implanted roots in experimental groups compared with controls. Vascular lumens were also observed in periodontal compartments of HUVEC-containing groups. Periodontal ligament regeneration, cementogenesis and osteogenesis were evident in the experimental groups at both weeks 4 and 8, as shown by immunostaining for periostin and bone sialoprotein. Human cells in the transplanted cell sheets were stained by immunohistochemistry for the presence of human mitochondria.ConclusionsThe 3D cell sheet-based approach may be potentially beneficial and is thus encouraged for future regenerative periodontal therapy.