Effects of intrathecal isoflurane administration on nociception and Fos expression in the rat spinal cord
Effects of intrathecal isoflurane administration on nociception and Fos expression in the rat spinal cord
复制标题
鞘内注射异氟醚对大鼠脊髓伤害感受和 Fos 表达的影响
DOI：
10.1097/eja.0b013e328340514a
复制
发表时间：
2011-02-01
影响因子：
3.6
通讯作者：
Xu, Li-Xian
中科院分区：
文献类型：
--
作者：
Liu, Chun-Ran;Duan, Qiao-Zhi;Xu, Li-Xian
背景与目的异氟醚作为吸入麻醉药已有近30年的历史。在麻醉期间吸入异氟烷也产生抗伤害感受作用。这是否发生在脊柱或脊髓上水平仍然未知。本研究采用一种新型液体异氟醚，观察了鞘内注射异氟醚对大鼠脊髓伤害性反应和Fos表达的影响。方法36只大鼠随机分为3组：A组（n = 6），鞘内注射生理盐水50 μl kg−1; B组和C组（n = 6），鞘内注射异氟醚25 μl kg−1和50 μl kg−1。在鞘内注射异氟烷后，对后爪施加伤害性热刺激（Hargreaves试验）、机械刺激（von Frey试验）和化学刺激（5%福尔马林，50 μl）以研究其抗伤害性效应。采用免疫组化和实时荧光定量PCR方法检测脊髓背角Fos蛋白和c-fos mRNA的表达。结果与生理盐水对照组相比，鞘内注射异氟醚可明显抑制福尔马林诱导的大鼠自发性缩足反应以及热刺激和机械刺激诱导的大鼠缩足反应，且呈剂量依赖性。免疫组织化学和实时逆转录PCR显示，异氟烷给药抑制福尔马林注射诱导的脊髓c-fos表达。结论异氟醚可通过抑制脊髓神经元的激活而发挥抗伤害性效应。
Background and objective Isoflurane has been used as an inhaled anaesthetic for nearly 30 years. Isoflurane inhalation during anaesthesia also produces an anti-nociceptive effect. Whether this occurs at the spinal or supraspinal level remains unknown. With a novel type of liquid isoflurane, the present study examined the effects of intrathecal isoflurane on the nociceptive response and Fos expression in the rat spinal cord. Methods Thirty-six rats were randomly assigned to three groups as follows: group A (n = 6), intrathecal physiological saline 50 μl kg−1; and group B and C (n = 6 each), intrathecal isoflurane at doses of 25 μl kg−1 or 50 μl kg−1, respectively. Noxious thermal (Hargreaves test), mechanical (von Frey test) and chemical (formalin 5%, 50 μl) stimuli were applied to a hind paw after intrathecal isoflurane injection to study its anti-nociceptive effect. In addition, the expression of Fos protein and c-fos mRNA in the spinal dorsal horns was detected by immunohistochemistry and real-time reverse transcriptase PCR, respectively. Results Compared with the physiological saline control, intrathecal isoflurane significantly suppressed spontaneous paw flinches in rats induced by formalin injection and paw withdrawal induced by thermal and mechanical stimuli in a dose-dependent manner. Immunohistochemistry and real-time reverse transcriptase PCR revealed that isoflurane administration inhibited formalin injection-induced c-fos expression in the spinal cord. Conclusions These data suggest that isoflurane can exert anti-nociceptive effects at the spinal level by preventing neuronal activation.