Tn10 and IS10 transposition and chromosome rearrangements: mechanism and regulation in vivo and in vitro.
Tn10 and IS10 transposition and chromosome rearrangements: mechanism and regulation in vivo and in vitro.
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Tn10 和 IS10 转座和染色体重排：体内和体外机制和调节。
DOI：
10.1007/978-3-642-79795-8_3
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发表时间：
1996
影响因子：
--
通讯作者：
Bolland,S
中科院分区：
文献类型：
--
作者：
Kleckner,N;Chalmers,RM;Kwon,D;Sakai,J;Bolland,S
Tn 10是一个复合转座子。它包含一对IS 10插入序列，位于约6.7 kb独特序列两侧的相反方向;这些独特序列编码四环素抗性决定簇和其他功能尚待鉴定的决定簇（图1A; Kleckner 1989）。Tn 10的两个IS 10元件之一，IS 10-Right，在结构和功能上是完整的，被认为是“野生型”IS 10。IS 10编码一个转座酶蛋白，它通过与两个相反方向的IS 10（或Tn 10）末端的特定序列相互作用来介导转座。IS 10的末端略有不同，根据它们在Tn 10中的位置，分别称为“外”端和“内”端。IS 10-Left结构完整，但编码一个实质上有缺陷的转座酶。
Tn10 is a composite transposon. It comprises a pair of IS10 insertion sequences located in opposite orientation flanking ~6.7 kb of unique sequences; these unique sequences encode a tetracycline resistance determinant and other determinants whose functions remain to be identified (Fig. 1A; Kleckner1989). One of Tn10’s two IS10 elements, IS10-Right, is structurally and functionally intact and is considered to be the “wild type” IS10. IS10 encodes a single transposase protein which mediates transposition by interacting with specific sequences at two oppositely oriented IS10 (or Tn10) termini. The termini of IS10 are subtly different and are referred to as the “outside” and “inside” end, respectively, by virtue of their position in Tn10. IS10-Left is structurally intact but encodes a substantially defective transposase.