BILIARY TRANSPORT OF IGA - ROLE OF SECRETORY COMPONENT
BILIARY TRANSPORT OF IGA - ROLE OF SECRETORY COMPONENT
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DOI：
10.1073/pnas.76.4.2008
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
UNDERDOWN, BJ
中科院分区：
文献类型：
--
作者：
FISHER, MM;NAGY, B;UNDERDOWN, BJ
用含放射性标记IG的血液灌流离体大鼠肝脏，研究了大鼠免疫球蛋白（IG）的胆汁转运。运输到胆汁是选择性的聚合IgA。在210分钟的灌注期间，15%至27%的聚合IgA从血液转运至胆汁，并且约60%的转运至胆汁的IgA具有分泌成分。运输少量IgM（0.12%）;未检测到IgG2a、IgE或单体IgA的运输。通过在人分泌组分-琼脂糖上的亲和层析纯化放射性标记的聚合IgA产生了更有效地转运的级分（即，40%以上）。分泌型IgA（初乳或胆汁）转运1/25 - 1/12，以及多聚型IgA骨髓瘤蛋白。125I-标记的分泌成分和聚合物IgA在体外形成的复合物运输差（0.1%）相比，聚合物IgA（26%）。聚合IgA的胆汁转运可能需要它与肝脏中的分泌成分相结合。兔IgG抗大鼠分泌成分抗体被转运到胆汁中，而正常兔IgG不被转运。
Biliary transport of rat immunoglobulin (Ig) was studied by perfusion of isolated rat liver with blood containing radiolabeled Ig. Transport to bile was selective for polymeric IgA. Between 15 and 27% of polymeric IgA was transported from blood to bile during a 210 min perfusion period and approximately 60% of the IgA transported to bile bore a secretory component. Small quantities of IgM (0.12%) were transported; transport of IgG2a, IgE or monomeric IgA was not detected. Purification of radiolabeled polymeric IgA by affinity chromatography on human secretory component-Sepharose yielded a fraction that was transported more efficiently (i.e., up to 40% transported). Secretory IgA (colostral or biliary) was transported 1/25th-1/12th as well as polymeric IgA myeloma protein. Complexes of 125I-labeled secretory component and polymeric IgA formed in vitro were transported poorly (0.1%) compared to polymeric IgA (26%). Biliary transport of polymeric IgA may require a combination of it with secretory component in the liver. Rabbit IgG anti-rat secretory component antibodies were transported to bile but normal rabbit IgG was not.