In vitro antimicrobial activity of propolis dry extract
In vitro antimicrobial activity of propolis dry extract
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DOI：
10.1179/joc.2000.12.5.390
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发表时间：
2000-10-01
影响因子：
1.8
通讯作者：
Gismondo, MR
中科院分区：
文献类型：
--
作者：
Drago, L;Mombelli, B;Gismondo, MR
在本研究中，研究了蜂胶这一蜜蜂的天然产物对不同病原体的抗菌和抗真菌性能。按NCCLS标准对金黄色葡萄球菌、A组β-溶血性链球菌、肺炎链球菌、卡他莫拉氏菌、流感嗜血杆菌、肺炎克雷伯菌、大肠埃希菌、奇异变形杆菌、铜绿假单胞菌、白色念珠菌等320株细菌进行最低抑菌浓度(MIC)和最低杀菌浓度(MBCs)测定，测定0、3、6、2、4倍的最低抑菌浓度(MIC)和最低杀菌浓度(MBCs)。蜂胶对大多数分离株具有良好的抗菌活性，尤其是肺炎链球菌、流感嗜血杆菌和卡他分支杆菌，但对肠杆菌科细菌无明显抑菌作用。时间-杀灭曲线显示蜂胶的抑菌活性而不是杀菌活性，后者只有在高浓度时才明显。
In this study the antibacterial and antifungal properties of propolis, a natural product of bees, have been investigated against different pathogens. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were determined according to NCCLS standards on 320 strains including Staphylococcus aureus, Group A beta-hemolytic streptococci, Streptococcus pneumoniae, Moraxella catarrhalis, Haemophilus influenzae, Klebsiella pneumoniae, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa and Candida albicans, Time-kill curves were assessed for susceptible microorganisms, testing 0, 0.5, 1, 2, 4 x MIC for propolis, by counting viable bacteria after 0, 3, 6, 24 hours and viable yeasts after 0, 3, 6, 24 and 48 hours. Propolis showed good antimicrobial activity against most of the isolates, particularly S. pneumoniae, H. influenzae and M. catarrhalis, but not against Enterobacteriaceae. Time-kill curves demonstrated bacteriostatic rather than bactericidal activity of propolis, the Latter being evident only at high concentrations.