Lymphocyte phenotyping and NK cell activity analysis in pregnant NOD/SCID mice
Lymphocyte phenotyping and NK cell activity analysis in pregnant NOD/SCID mice
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DOI：
10.1016/j.jri.2005.05.002
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发表时间：
2005-12-01
影响因子：
3.4
通讯作者：
Zeng, S
中科院分区：
文献类型：
--
作者：
Lin, Y;Chen, YJ;Zeng, S
本研究的目的是概述非肥胖糖尿病（NOD）/LtSz-scid/scid（NOD/SCID [严重联合免疫缺陷]简称）小鼠的生育特征，并评估NK细胞亚群对同基因NOD/SCID × NOD/SCID交配组合妊娠结局的影响。首先，用流式细胞仪进行淋巴细胞表型分析以证实NOD/SCID小鼠的多重免疫缺陷。评估了NOD/SCID x NOD/SCID小鼠的生育力特征，并与非免疫缺陷对照BALB/c x BALB/c小鼠进行了比较。虽然NK细胞缺陷的存在在NOD/SCID小鼠中是明显的，但在这些小鼠中可以观察到一定水平的残余NK活性。用聚肌胞苷酸（polyinosinic-polycytidylic acid，polyIC）刺激残存NK细胞活性，用抗去唾液酸GM 1（asialo GM 1，ASGM 1）抗血清抑制残存NK细胞活性。给药后评价这些因素对妊娠结局的影响。在NOD/SCID x NOD/SCID小鼠中可以观察到大致正常的生育力。然而，与对照NOD/SCID小鼠相比，在polyIC处理的NOD/SCID x NOD/SCID小鼠中观察到略大尺寸的窝仔。相比之下，ASGM 1注射后，胚胎吸收增强，随后与较小的窝仔数相关。提示NOD/SCID小鼠体内残存的NK细胞活性可能有利于妊娠期母胎耐受。(C)2005爱思唯尔爱尔兰有限公司保留所有权利。
The purpose of this study was to outline the fertility features of non-obese diabetic (NOD)/LtSz-scid/scid (NOD/SCID [severe combined immunodeficiency] for short) mice, and to evaluate the effects of NK cell subsets on the pregnancy outcomes of the syngeneic NOD/SCID x NOD/SCID mating combination. Firstly, lymphocyte phenotyping was performed with flow cytometry to confirm the multiple immunodeficits in NOD/SCID mice. Fertility features were assessed in NOD/SCID x NOD/SCID mice and compared with non-immunodeficiency control BALB/c x BALB/c mice. Although the presence of NK cell deficit is apparent in NOD/SCID mice, a certain level of remnant NK activity could be observed in these mice. The remnant NK cell activity was stimulated with polyinosinic-polycytidylic acid (polyIC), or inhibited with anti-asialo GM 1 (ASGM1) anti-serum, respectively. The effects of these factors on pregnancy outcomes were evaluated after administration. Roughly normal fertility could be observed in NOD/SCID x NOD/SCID mice. However, a slightly larger sized litter was observed in polyIC-treated NOD/SCID x NOD/SCID mice compared with control NOD/SCID mice. In contrast, embryo resorption was boosted after ASGM1 injection, and correlated subsequently with a smaller litter size. It indicates that the remnant NK cell activity in NOD/SCID mice may be beneficial to feto-maternal tolerance during pregnancy. (C) 2005 Elsevier Ireland Ltd. All rights reserved.