Oxidative stress parameters in rats exposed to fluoride and caffeine
Oxidative stress parameters in rats exposed to fluoride and caffeine
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DOI：
10.1016/j.fct.2010.03.033
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发表时间：
2010-06-01
影响因子：
4.3
通讯作者：
Czarnowski, W.
中科院分区：
文献类型：
--
作者：
Inkielewicz-Stepniak, I.;Czarnowski, W.
本实验观察了咖啡因（Caff）和氟（F）单独和联合染毒1个月后对大鼠血清、脑、肝、肾组织中一氧化氮（NO）和总抗氧化状态的影响。并研究了咖啡因对尿氟排泄的影响。将30只成年雄性Wistar大鼠分成5个相等的组，每组6只：（I）对照组饮用自来水;（II）对照组饮用自来水并灌胃接受0.5 ml自来水;（III）动物接受25 mg F/L的饮用水;（IV）动物接受4.7 mg Caff/kg bw/天;（V）动物在饮用水中接受25 mg F/L和4.7 mg Caff/kg bw/天。结果表明，氟暴露可引起血清、脑、肾、肝组织中一氧化氮（NO）水平升高，脂质过氧化反应（TBARS）加剧，总抗氧化状态下降。咖啡因作为一种抗氧化剂灌胃给药，在减轻F.氟处理组大鼠尿氟排泄量显著增加，且呈时间依赖性，而咖啡因处理组和咖啡因与氟联合处理组大鼠尿氟排泄量无明显变化。(C)2010爱思唯尔有限公司版权所有。
In our experiment, the 1-month effects of caffeine (Caff) and fluoride (F) administered separately and together on nitric oxide and total antioxidant status in serum, brain, liver and kidney of rats were investigated. Also, the influence of caffeine on fluoride excretion with urine was studied. Thirty adult male Wistar rats were divided into five equal groups of six each: (I) controls drinking tap water; (II) controls drinking tap water and receiving intragastrically 0.5 ml of tap water; (III) animals receiving 25 mg F/L in drinking water; (IV) animals receiving 4.7 mg Caff/kg bw/day; (V) animals receiving 25 mg F/L in drinking water and 4.7 mg Caff/kg bw/day. The applied fluoride caused increase of nitric oxide level (NO), intensified lipid peroxidation (TBARS) and decreased total antioxidant status in serum (TAS), brain, kidney and liver. Caffeine administered intragastrically, as an antioxidant, was relatively efficient in alleviating these adverse effects of F. In rats treated only with fluoride the F excretion in urine significantly increased in an exposure-time dependent-manner and did not change both in rats treated with Caff and co-exposed to Caff and F. (C) 2010 Elsevier Ltd. All rights reserved.