LINKAGE OF KDR-TYPE RESISTANCE AND THE PARA-HOMOLOGOUS SODIUM-CHANNEL GENE IN GERMAN COCKROACHES (BLATTELLA-GERMANICA)
LINKAGE OF KDR-TYPE RESISTANCE AND THE PARA-HOMOLOGOUS SODIUM-CHANNEL GENE IN GERMAN COCKROACHES (BLATTELLA-GERMANICA)
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DOI：
10.1016/0965-1748(94)90051-5
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发表时间：
1994-07-01
影响因子：
3.8
通讯作者：
SCOTT, JG
中科院分区：
文献类型：
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作者：
DONG, K;SCOTT, JG
拟除虫菊酯是一类重要的杀虫剂，用于控制害虫，包括德国蟑螂。不幸的是，许多昆虫已经对拟除虫菊酯产生了抗药性。最重要的抗性机制之一是KDR(基因敲除抗性)，其特征是神经对拟除虫菊酯和DDT不敏感。为了研究电压依赖性钠通道是否参与德国蟑螂的kdr型抗性，我们从德国蟑螂中克隆了一段120bp的类同源钠通道基因的DNA片段。以该片段为探针，我们在敏感和kdr型抗性的德国蟑螂中发现了类同源钠通道基因的限制性片段长度多态性(RFLP)。对F-2和回交蟑螂群体(共331只)的RFLP分析表明，所有纯合子抗性个体都有3.7kb的EcoRI片段，所有纯合子敏感个体都有3.0kb的EcoRI片段，所有杂合子个体都有3.7和3.0kb的片段。未检测到KDV型抗性基因与类同源钠通道基因的重组。这表明KDR型抗性基因与类同源钠通道基因是相同的或紧密连锁的(
Pyrethroids are an important class of insecticides for controlling insect pests, including the German cockroach. Unfortunately, many insects have developed resistance to pyrethroids. One of the most important mechanisms of resistance is kdr (knockdown resistance) which is characterized by neural insensitivity to pyrethroids and DDT. To investigate whether the voltage-dependent sodium channel is involved in kdr-type resistance in the German cockroach, we isolated a 120 bp DNA fragment of the para-homologous sodium channel gene from German cockroaches. Using this fragment as a probe, we identified a restriction fragment length polymorphism(RFLP) of the para-homologous sodium channel gene between susceptible and kdr-type resistant German cockroaches. RFLP analysis of F-2 and backcross cockroach populations (total of 331 individuals) showed that all homozygous resistant individuals had a 3.7 kb EcoRI fragment, all homozygous susceptible individuals had a 3.0 kb EcoRI fragment, and all heterozygous individuals had both 3.7 and 3.0 kb fragments. No recombination was detected between the kdv-type resistance locus and the para-homologous sodium channel gene. This suggests that the kdr-type resistance locus and para-homologous sodium channel gene are identical or tightly linked (