Differentiation of a mouse submandibular gland-derived cell line (SCA) grown on matrigel
Differentiation of a mouse submandibular gland-derived cell line (SCA) grown on matrigel
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DOI：
10.1016/j.yexcr.2005.04.025
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发表时间：
2005-08-15
影响因子：
3.7
通讯作者：
Miyazaki, Y
中科院分区：
文献类型：
--
作者：
Barka, T;Gresik, ES;Miyazaki, Y
从小鼠颌下腺诱导肿瘤中培养出SCA-9细胞系(T. Barka， H. van der Noen， A.M.Michelakis， I. Schenkein，表皮生长因子，肾素和肽酶在培养的颌下腺肿瘤细胞中的作用。实验室。投资。， 42(1980) 656-662)。我们研究了SCA细胞在不同基质上培养的一些表型特征。在塑料表面，细胞呈单层生长；在矩阵上，它们形成分支结构和管状结构，这种现象称为分支形态发生。EGF和HGF促进细胞生长和分支形态的形成，而这种作用被抗EGF抗体抑制。我们对生长在塑料表面或基质上的细胞进行了RT-PCR和实时定量RT-PCR。生长在塑料上的细胞表达EGF和肾素2，但没有或只有微量的NGF。在基质上生长24小时，EGF mRNA瞬间增加21倍，肾素2 mRNA瞬间增加3371倍。NGF mRNA水平无明显变化。SCA-9细胞表达EGF家族配体受体的mrna。在塑料上，主要表达ErbB1和ErbB2。基质培养导致ErbB1和ErbB2 mRNA水平分别增加了11倍，ErbB3和ErbB4 mRNA水平分别增加了221倍和85倍。小干扰rna siErbB3和siErbB4抑制生长在塑料或基质上的细胞的生长。siErbB1 + siErbB3和siErbB2 + siErbB3也有明显的生长抑制作用。siErbB1和siErbB2也抑制分支形态发生。由于SCA细胞表达EGF和EGF受体，EGF在促进生长和分支形态发生中起自分泌调节作用。我们认为SCA细胞提供了一个有用的模型来分析分支形态发生的机制和基质在调节培养细胞表型特征表达中的作用。(c) 2005爱思唯尔公司版权所有。
SCA-9 cell line was developed from an induced tumor of mouse submandibular gland (T. Barka, H. van der Noen, A.M. Michelakis, I. Schenkein, Epidermal growth factor, renin, and peptidase in cultured tumor cells of submandibular gland origin. Lab. Invest., 42 (1980) 656-662). We have studied some of the phenotypic characteristics of SCA cells cultured on different matrices. On plastic surface, the cells grow as a monolayer; on matrigel, they form branching structures and tubes, a phenomenon termed branching morphogenesis. EGF and HGF promoted cellular growth and branching morphogenesis which was inhibited by anti-EGF antibodies. We have performed RT-PCR and real-time quantitative RT-PCR of cells grown on plastic surface or on matrigel. Grown on plastic, the cells express EGF and renin 2, but no or only trace amounts of NGF. Growth on matrigel for 24 h resulted in a transient 21-fold increase in EGF mRNA and a 3371-fold increase in renin 2 mRNA. There was no change in NGF mRNA level. SCA-9 cells express mRNAs for receptors for the EGF family of ligands. On plastic, mainly ErbB1 and ErbB2 are expressed. Culture on matrigel resulted in 11-fold increase in mRNA levels for ErbB1 and ErbB2, and a 221-fold and 85-fold increase in the mRNA levels for ErbB3 and ErbB4, respectively. Small interfering RNAs siErbB3 and siErbB4 inhibited the growth of the cells grown on plastic or matrigel. Significant growth inhibition was seen also with siErbB1 + siErbB3 and siErbB2 + siErbB3. siErbB1 and siErbB2 also inhibited branching morphogenesis. Since SCA cells express EGF and receptors for EGF, EGF acts an autocrine regulator in promoting growth and branching morphogenesis. We conclude that SCA cells provide a useful model to analyze the mechanism of branching morphogenesis and the role of matrix in regulating expression of phenotypic characteristics of cultured cells. (c) 2005 Elsevier Inc. All rights reserved.