Tissue-Specific Control of the Endocycle by the Anaphase Promoting Complex/Cyclosome Inhibitors UVI4 and DEL1
Tissue-Specific Control of the Endocycle by the Anaphase Promoting Complex/Cyclosome Inhibitors UVI4 and DEL1
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DOI：
10.1104/pp.17.00785
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发表时间：
2017-09-01
期刊：
影响因子：
7.4
通讯作者：
De Veylder, Lieven
中科院分区：
文献类型：
--
作者：
Heyman, Jefri;Polyn, Stefanie;De Veylder, Lieven
内环代表着一种经过修饰的有丝分裂细胞周期，在植物中，这种周期通常与细胞的扩大和分化有关。内周期的起始是由后期促进复合体/环体(APC/C)的活性控制的，APC/C是一种多亚单位E3泛素连接酶，靶向细胞周期因子进行破坏。细胞周期蛋白SWITCH52(CCS52)代表APC/C的限速激活子亚基。在拟南芥中，CCS52A1或CCS52A2激活子的突变会导致内周期的延迟，而它们的过度表达则会触发DNA倍体水平的增加。在这里，通过分析APC/C-CCS52A1和APC/C-CCS52A2复合体的负调控因子，分别是UV-B-INSENSITIVE4蛋白和DP-E2F-like 1转录抑制因子，研究了APC/C-CCS52A1和APC/C-CCS52A2复合体在不同发育过程中的相对贡献。我们的数据说明了APC/C-CCS52A1和APC/C-CCS52A2复合体在根和毛状体发育过程中的协同活性，但在叶发育过程中功能上的相互依赖。此外，我们还发现APC/C-CCS52A1活性可以控制CCS52A2的表达。我们得出结论，CCS52A控制的APC/C活性的相互依赖性是以组织特异性的方式控制的。
The endocycle represents a modified mitotic cell cycle that in plants is often coupled to cell enlargement and differentiation. Endocycle onset is controlled by activity of the Anaphase Promoting Complex/Cyclosome (APC/C), a multisubunit E3 ubiquitin ligase targeting cell-cycle factors for destruction. CELL CYCLE SWITCH52 (CCS52) proteins represent rate-limiting activator subunits of the APC/C. In Arabidopsis (Arabidopsis thaliana), mutations in either CCS52A1 or CCS52A2 activators result in a delayed endocycle onset, whereas their overexpression triggers increased DNA ploidy levels. Here, the relative contribution of the APC/C-CCS52A1 and APC/C-CCS52A2 complexes to different developmental processes was studied through analysis of their negative regulators, being the ULTRAVIOLET-B-INSENSITIVE4 protein and the DP-E2F-Like1 transcriptional repressor, respectively. Our data illustrate cooperative activity of the APC/C-CCS52A1 and APC/C-CCS52A2 complexes during root and trichome development, but functional interdependency during leaf development. Furthermore, we found APC/C-CCS52A1 activity to control CCS52A2 expression. We conclude that interdependency of CCS52A-controlled APC/C activity is controlled in a tissue-specific manner.