A Young Blood Environment Decreases Aging of Senile Mice Kidneys
A Young Blood Environment Decreases Aging of Senile Mice Kidneys
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年轻的血液环境可减少老年小鼠肾脏的衰老
DOI：
10.1093/gerona/glx183
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发表时间：
2018-04-01
影响因子：
5.1
通讯作者：
Chen, Xiangmei
中科院分区：
文献类型：
--
作者：
Huang, Qi;Ning, Yichun;Chen, Xiangmei
体内环境的变化是否会影响肾脏的衰老仍不清楚。具体来说，尚不清楚来自老年供体的移植肾脏在植入年轻受体后是否会从组织损伤中恢复。本研究建立了一种联体动物模型，探讨了年轻体内环境对老年肾脏的影响。动物分为6组：青年对照组（Ycon）、老年对照组（Ocon）、等时青年-青年组（Y-IP）、老年-老年组（O-IP）、异时青年组（Y-HP）和老年组（O-HP）。联体生活后，O-HP组的肾小管和间质组织评分显著低于Ocon和O-IP组。O-HP组衰老相关蛋白p16和SA-β-gal的表达较Ocon组和O-IP组明显降低。与Ocon和O-IP组相比，O-HP组中自噬因子Atg 5和LC 3BII的表达显著上调，而自噬降解标记物（P62）的表达显著下调。O-HP组TUNEL染色阳性细胞数、IL-6和IL-1β表达明显减少，caspase-3和NF-κB表达明显增加。结果表明，年轻的血液环境显着降低肾脏衰老。这些发现提供了新的证据，支持提高人类肾移植供体的年龄上限。
Whether changes in internal body environment affect kidney aging remains unclear. Specifically, it is unknown whether transplanted kidneys from older donors recover from tissue damage after placement in younger recipients. In this study, a parabiosis animal model was established to investigate the effects of a young internal body environment on aged kidneys. The animals were divided into six groups: young (Ycon) and old control (Ocon) groups, isochronic youth-youth group (Y-IP), elderly-elderly group (O-IP), and heterochronic youth (Y-HP) and elderly (O-HP) groups. After parabiosis, tubule and interstitial tissue scores in the O-HP group were significantly lower than in the Ocon and O-IP groups. The expression of aging-related protein p16 and SA-β-gal in the O-HP group was significantly reduced compared with the Ocon and O-IP groups. Autophagy factors Atg5 and LC3BII were significantly upregulated, whereas the expression of the autophagic degradation marker (P62) was significantly downregulated in the O-HP group compared with the Ocon and O-IP groups. With the same comparison, the positive cells of TUNEL staining and the expression of IL-6 and IL-1β were significantly reduced, whereas the total/cleaved caspase-3 and total/pNF-κB were significantly increased in the O-HP group. The results demonstrated that a young blood environment significantly reduces kidney aging. These findings provide new evidence supporting an increase in the upper age limit for human kidney transplantation donors.