Pore formation in a binary giant vesicle induced by cone-shaped lipids.
Pore formation in a binary giant vesicle induced by cone-shaped lipids.
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锥形脂质诱导的二元巨型囊泡中的孔形成。
DOI：
10.1016/j.bpj.2010.03.064
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发表时间：
2010
影响因子：
3.4
通讯作者：
M. Imai
中科院分区：
文献类型：
--
作者：
Y. Sakuma;T. Taniguchi;M. Imai
我们研究了由圆锥形和圆柱形脂质组成的二元巨单层囊泡（GUV）的形状变形。通过将脂质的自发曲率与相分离耦合，我们证明了GUV中的孔打开和关闭。当温度设定为低于圆柱形脂质的链熔融转变温度时，GUV破裂，然后形成单个大孔，其中圆锥形脂质在双层的边缘形成帽以稳定孔。当温度升高到相变温度以上时，孔闭合。根据锥形脂质浓度的不同，孔呈现出三种类型的形状：简单圆形孔、卷边孔和卷边孔。这些孔形状的变化表明，在双层的内部和外部小叶之间的圆锥形和圆柱形的脂质的分布是不对称的。我们提出了一个理论模型的双组分膜的边缘双层脂质可以转移之间的两个小叶。利用这个模型，我们数值再现了观察到的形状变形的孔的边缘。
We have investigated shape deformations of binary giant unilamellar vesicles (GUVs) composed of cone- and cylinder-shaped lipids. By coupling the spontaneous curvature of lipids with the phase separation, we demonstrated pore opening and closing in GUVs. When the temperature was set below the chain melting transition temperature of the cylinder-shaped lipid, the GUVs burst and then formed a single large pore, where the cone shape lipids form a cap at the edge of the bilayer to stabilize the pore. The pore closed when we increased the temperature above the transition temperature. The pore showed three types of shapes depending on the cone-shaped lipid concentration: simple circular, rolled-rim, and wrinkled-rim pores. These pore shape changes indicate that the distribution of the cone- and cylinder-shaped lipids is asymmetric between the inner and outer leaflets in the bilayer. We have proposed a theoretical model for a two-component membrane with an edge of bilayer where lipids can transfer between two leaflets. Using this model, we have reproduced numerically the observed shape deformations at the rim of pore.
影响因子：
2.9
作者：
SANDERS, CR;SCHWONEK, JP
通讯作者：
SCHWONEK, JP
影响因子：
3.3
作者：
Lipfert, Jan;Columbus, Linda;Doniach, Sebastian
通讯作者：
Doniach, Sebastian