Microwave preservation method for DMSP, DMSO, and acrylate in unfiltered seawater and phytoplankton culture samples
Microwave preservation method for DMSP, DMSO, and acrylate in unfiltered seawater and phytoplankton culture samples
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DOI：
10.1002/lom3.10081
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发表时间：
2016-03
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影响因子：
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通讯作者：
J. D. Kinsey;D. Kieber
中科院分区：
文献类型：
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作者：
J. D. Kinsey;D. Kieber
建立了一种微波保存方法来定量未过滤样品中的总二甲基磺酸丙酸酯(DMSPT)、二甲基亚砜(DMSOT)和丙烯酸酯(丙烯酸酯T)浓度，以缓解酸化方法应用于含棕囊藻样品时的相关问题。用南极棕囊藻和其他11种海洋浮游植物的间歇培养法进行DMSPT，南极棕囊藻和丙烯酸酯T的间歇培养，以及特拉华州河口水样的DMSPT进行微波和酸保存方法的比较，以证明这种方法的普遍适用性。南极冰藻养殖样品的酸化导致DMSPT(42-69%)被低估，而二甲基硫(DMS)(2156-3819%)、DMSOT(9-101%)和丙烯酸酯T(71-249%)被高估。通过比较，微波处理的样品中的DMSPT浓度与非微波处理、非酸化的对照样品一致。与南极洲的结果相比，微波和酸保存方法得到的DMSPT浓度在统计上无法区分其他11种海洋浮游植物和特拉华州河口样本。未过滤的样品在微波处理后冷冻或在室温下储存，如果在微波步骤后酸化，则不会导致DMSPT或丙烯酸酯T的变化；当在酸化和室温储存之前不进行喷雾以去除二甲基硫时，DMSOT浓度略有增加(∼15%)。基于这些发现，我们建议微波处理小体积(≤7毫升)的未过滤海水或养殖样品作为一种通用的方法来保存样品，用于后续的DMSPT、DMSOT和丙烯酸酯T分析，特别是在样品的浮游植物组成未知的情况下。
A microwave‐preservation method was developed to quantify total dimethylsulfoniopropionate (DMSPT), dimethylsulfoxide (DMSOT), and acrylate (acrylateT) concentrations in unfiltered samples to alleviate problems associated with the acidification method when applied to samples containing Phaeocystis. Microwave‐ and acid‐preservation methods were compared using batch cultures of Phaeocystis antarctica and 11 other marine phytoplankton species for DMSPT, batch P. antarctica cultures for DMSOT and acrylateT, and unfiltered Delaware Estuary water samples for DMSPT to demonstrate the general applicability of this method. Acidification of P. antarctica culture samples resulted in the underestimation of DMSPT (42–69%) and overestimation of dimethylsulfide (DMS) (2156–3819%), DMSOT (9–101%), and acrylateT (71–249%). By comparison, DMSPT concentrations in microwaved samples agreed with non‐microwaved, non‐acidified controls. In contrast to P. antarctica results, the microwave‐ and acid‐preservation methods yielded DMSPT concentrations that were statistically indistinguishable for 11 other marine phytoplankton species and Delaware Estuary samples. Unfiltered samples stored frozen following microwave treatment or stored at room temperature if acidified after the microwaving step, resulted in no change in DMSPT or acrylateT; DMSOT concentrations increased slightly (∼ 15%) when they were not sparged to remove DMS prior to acidification and room temperature storage. Based on these findings, we propose microwaving small sample volumes (≤ 7 mL) of unfiltered seawater or culture samples as a general approach to preserve samples for subsequent DMSPT, DMSOT, and acrylateT analyses, especially when the phytoplankton composition of the samples is unknown.