Dental Pulp Dendritic Cells Migrate to Regional Lymph Nodes
Dental Pulp Dendritic Cells Migrate to Regional Lymph Nodes
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DOI：
10.1177/0022034513518223
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发表时间：
2014-03-01
影响因子：
7.6
通讯作者：
Azuma, M.
中科院分区：
文献类型：
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作者：
Bhingare, A. C.;Ohno, T.;Azuma, M.
树突状细胞（DC）迁移至区域淋巴结（RLN）是适应性免疫的重要步骤，迁移的DC上的细胞表面抗原极大地影响后续免疫反应的质量和数量。尽管牙髓中存在 MHC II 类+ DC 样细胞，但这些细胞的谱系和功能仍然未知。在这里，我们在 KikGR 小鼠的牙尖修剪和酸蚀刻后从牙髓中鉴定出迁移性 DC，其中光转换荧光蛋白在紫光照射下从绿色变为红色。尖牙处理后 16 小时，来自牙髓的两个主要细胞部分已迁移至 RLN，这在主要部分和第二部分中显示出以下谱系标记：CD11c（高）CD11b（++）Ly6C（低）Ly6G（低）F4/80（+）和分别为CD11c(med)CD11b(+++)Ly6C(++)Ly6G(+++)F4/80(-)。这些谱系标记表明，前者细胞是通过传入淋巴管迁移的DC，后者是通过血液循环招募的粒细胞。迁移的牙髓 DC 成熟，表达最高水平的 CD273 (B7-DC) 和 CD86 共刺激剂以及 MHC II 类。我们的结果表明，暴露于致龋细菌的牙髓 DC 会迁移到 RLN，并在那里触发适应性免疫反应。
Dendritic cell (DC) migration to regional lymph nodes (RLNs) is an essential step in adaptive immunity, and cell-surface antigens on migrating DCs greatly affect the quality and quantity of subsequent immune responses. Although MHC class II+ DC-like cells exist in the dental pulp, the lineage and function of these cells remain unknown. Here, we identified migratory DCs from the dental pulp after cusp trimming and acid etching in KikGR mice, in which the photoconvertible fluorescent protein changed from green to red upon violet light exposure. Two major cell fractions from the dental pulp had migrated to the RLNs at 16 hrs after cusp treatment, which showed the following lineage markers in the main and second fractions: CD11c(high)CD11b(++)Ly6C(low) Ly6G(low) F4/80(+) and CD11c(med)CD11b(+++)Ly6C(++)Ly6G(+++)F4/80(-), respectively. These lineage markers indicate that the former cells were DCs that had migrated through afferent lymphoid vessels, and the latter were granulocytes recruited via blood circulation. Migratory dental pulp DCs were mature, expressing the highest levels of CD273 (B7-DC) and CD86 co-stimulators and MHC class II. Our results suggest that cariogenic-bacteria-exposed dental pulp DCs migrate to RLNs and there trigger adaptive immune responses.