Comparative study of photodissociation and surface-induced dissociation by laser desorption Fourier transform mass spectrometry.
Comparative study of photodissociation and surface-induced dissociation by laser desorption Fourier transform mass spectrometry.
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激光解吸傅里叶变换质谱法光解离和表面诱导解离的比较研究。
DOI：
10.1021/ac00043a010
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发表时间：
1992
影响因子：
7.4
通讯作者：
Wilkins,CL
中科院分区：
文献类型：
--
作者：
Castoro,JA;Nuwaysir,LM;Ijames,CF;Wilkins,CL
比较了激光解吸傅里叶变换质谱（LD/FTMS）分析几种非挥发性化合物的光解离（PD）和表面诱导解离（SID）。本文研究了一种卟啉和两种金属卟啉在不同实验条件下的SID和PD。当使用575-nm辐射对母离子照射相对长的时间（10-30 s）和使用308-或388-nm辐射照射较短的时间（0.5-1 s）时，从PD获得最佳结构信息。在可见光区较短的照射时间导致结构上重要的产物吨的生产效率较低，而在紫外区较长的时间产生更多的非特异性碎片离子，显然是以牺牲更多的结构上重要的碎片吨为代价。SID转换效率的卟啉估计碰撞能量从25至380 eV，与最大转换效率发现使用82-和115-eV的coHMon能量的两个卟啉研究。在这些结果的背景下，讨论了MS/MS/MS联合使用PD和SID的并行研究结果。由两种解离技术产生的MS 3离子谱比MS 2产物离子谱差异更显著。这些数据表明，采用PD和SID进行离子活化的LD/FTMS分析的非挥发性化合物的MS研究的一些一般准则。
Photodissociation (PD) and surface-induced dissociation (SID) are compared for structural analysis of several nonvolatile compounds analyzed by laser desorption Fourier transform mass spectrometry (LD/FTMS). SID and PD of a porphyrin and two metalloporphyrlns were Investigated using a variety of experimental conditions. Optimum structural Information Is obtained from PD when parent Ions are Irradiated for relatively long times (10-30 s) using 575-nm radiation and short times (0.5-1 s) using 308-or 388-nm radiation. Shorter Irradiation times In the visible region resulted In less efficient production of structurally significant product tons, while longer times In the ultraviolet region produced more nonspecific fragment ions, apparently at the expense of more structurally significant fragment tons. SID conversion efficiencies for the porphyrins are estimated for collision energies from25 to 380 eV, with maximum conversion efficiency found using 82-and 115-eV coHMon energies for the two porphyrins studied. Results from a concurrent study on the combined use of PD and SID for MS/MS/MS are discussed In the context of these results. The MS3 ton spectra generated by the two dissociation techniques differ more significantly than MS2 product Ion spectra. These data suggest some general guidelines for MS" studies of nonvolatile compounds analyzed by LD/FTMS, employing PD and SID for Ion activation.