A landscape of Long non-coding RNAs reveals the leading transcriptome alterations in murine aorta during aging.
A landscape of Long non-coding RNAs reveals the leading transcriptome alterations in murine aorta during aging.
复制标题
长链非编码 RNA 的景观揭示了衰老过程中小鼠主动脉中主要的转录组变化。
DOI：
10.1016/j.ygeno.2023.110573
复制
发表时间：
2023-02
期刊：
影响因子：
4.4
通讯作者：
Qi Liu
中科院分区：
文献类型：
--
作者：
Mengyue Yang;Meng Zhang;Zhaoying Li;Jingbao Liu;Yanchao Li;Ziyu Yang;Xuedong Wang;Xingtao Huang;Bo Yu;Jingbo Hou;Qi Liu
大量研究已经提供了令人信服的证据，证明血管衰老在预防心血管疾病方面处于领先地位。在衰老相关疾病中，长非编码RNA(LncRNAs)的各种功能正变得越来越明显。本研究旨在更好地了解lncRNAs在血管衰老中的表达谱和机制。用高通量测序技术检测96周龄和8周龄小鼠主动脉中lncRNAs和mRNAs的差异表达(DE)，同时检测到1423个lncRNAs和80mRNAs的差异表达。通过GO和KEGG分析，我们发现DE lncRNAs主要参与嘌呤代谢和cGMP-PKG信号通路。此外，还构建了一个DE lncRNAs和DE mRNAs共表达的功能网络，其中ENSMUST00000218874可以与41个DE mRNAs相互作用，提示它可能在维管衰老中起重要作用。本研究揭示了自然衰老血管中的DE lncRNAs，可能为老年心血管疾病的治疗提供新的思路和靶点。
Considerable studies have given convincing evidence of a forefront position for vascular aging in preventing cardiovascular disease. Various functions of Long non-coding RNAs (lncRNAs) are becoming increasingly distinct in aging-related diseases. This study aims at a better insight into the expression profile and mechanisms of lncRNAs in vascular senescence. High-throughput sequencing was used to detect the differential expression (DE) of lncRNAs and mRNAs in the aorta of 96 W and 8 W-old mice, while 1423 lncRNAs and 80 mRNAs were differentially expressed. By performing GO and KEGG enrichment analysis, we found that DE lncRNAs were mainly involved in purine metabolism and cGMP-PKG signaling pathways. In addition, a co-expression functional network of DE lncRNAs and DE mRNAs was constructed, and ENSMUST00000218874 could interact with 41 DE mRNAs, suggesting that it may play an essential role in vascular senescence. This study reveals DE lncRNAs in naturally aging vascular, which may provide new ideas and targets for aging-related cardiovascular diseases.