Fullerene C60 exposure elicits an oxidative stress response in embryonic zebrafish
Fullerene C60 exposure elicits an oxidative stress response in embryonic zebrafish
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DOI：
10.1016/j.taap.2007.12.030
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发表时间：
2008-05-15
影响因子：
3.8
通讯作者：
Tanguay, Robert L.
中科院分区：
文献类型：
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作者：
Usenko, Crystal Y.;Harper, Stacey L.;Tanguay, Robert L.
由于其独特的物理化学和光学特性，C-60 引起了人们对各种产品商业化的兴趣。虽然一些报告已经确定这种纳米材料可以作为一种强大的抗氧化剂，但许多其他研究已经证明通过光活化具有很强的氧化潜力。为了直接解决 C-60 的氧化潜力，评估了光和化学补充以及谷胱甘肽 (GSH) 消耗对 C-60 诱导的毒性的影响。使用胚胎斑马鱼作为模型生物来检查 C-60 引发氧化应激反应的潜力。在 C-60 暴露期间减少光照可显着降低死亡率以及鳍畸形和心包水肿的发生率（200 和 300 ppb C-60）。胚胎同时暴露于谷胱甘肽前体 N-乙酰半胱氨酸 (NAC)，也显示出死亡率和心包水肿的降低。然而，鳍畸形并没有减少。相反，同时暴露于 GSH 合成抑制剂丁硫氨酸亚磺酰亚胺 (BSO) 和马来酸二乙酯 (DEM) 会增加斑马鱼对 C-60 暴露的敏感性。 C-60 或其羟基化衍生物 C-60(OH)(24) 与 H2O2 共同暴露会导致两种材料的死亡率沿着 H2O2 浓度梯度增加。使用微阵列检测 C-60 在受精后 36 小时和 48 小时 (hpf) 这两个时间点对整体基因表达的影响。在两个生命阶段，几个关键应激反应基因的表达发生了变化，包括谷胱甘肽-S-转移酶、谷氨酸半胱氨酸连接酶、铁蛋白、α-生育酚转运蛋白和热休克蛋白70。这些结果支持C-60在该模型系统中诱导氧化应激的假设。 (C) 2008 Elsevier Inc. 保留所有权利。
Due to its unique physicochemical and optical properties, C-60 has raised interest in commercialization for a variety of products. While several reports have determined this nanomaterial to act as a powerful antioxidant, many other studies have demonstrated a strong oxidative potential through photoactivation. To directly address the oxidative potential of C-60, the effects of light and chemical supplementation and depletion of glutathione (GSH) on C-60-induced toxicity were evaluated. Embryonic zebrafish were used as a model organism to examine the potential of C-60 to elicit oxidative stress responses. Reduced light during C-60 exposure significantly decreased mortality and the incidence of fin malformations and pericardial edema at 200 and 300 ppb C-60. Embryos co-exposed to the glutathione precursor, N-acetylcysteine (NAC), also showed reduced mortality and pericardial edema; however, fin malformations were not reduced. Conversely, co-exposure to the GSH synthesis inhibitors, buthionine sulfoximine (BSO) and diethyl maleate (DEM), increased the sensitivity of zebrafish to C-60 exposure. Co-exposure of C-60 or its hydroxylated derivative, C-60(OH)(24), with H2O2 resulted in increased mortality along the concentration gradient of H2O2 for both materials. Microarrays were used to examine the effects of C-60 on the global gene expression at two time points, 36 and 48 h post fertilization (hpf). At both life stages there were alterations in the expression of several key stress response genes including glutathione-S-transferase, glutamate cysteine ligase, ferritin, a-tocopherol transport protein and heat shock protein 70. These results support the hypothesis that C-60 induces oxidative stress in this model system. (C) 2008 Elsevier Inc. All rights reserved.