In vivo visualization of hippocampal cells and dynamics of Ca2+ concentration during anoxia: Feasibility of a fiber optic plate microscope system for in vivo experiments
In vivo visualization of hippocampal cells and dynamics of Ca2+ concentration during anoxia: Feasibility of a fiber optic plate microscope system for in vivo experiments
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DOI：
10.1016/0006-8993(96)00487-8
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发表时间：
1996-09-02
期刊：
影响因子：
2.9
通讯作者：
Miyakawa, A
中科院分区：
文献类型：
--
作者：
Hirano, M;Yamashita, Y;Miyakawa, A
研究了采用光纤束和视频显微镜进行体内实验的光纤板（FOP）显微镜系统的可行性。这里使用的 FOP 由直径为 3 μm 的光纤组成。通过将 FOP 插入动物体内，可以以二维图像的形式获得来自表面不可见的深层组织的光学信号。使用该系统，可以对麻醉大鼠中用荧光染料染色的海马细胞进行可视化。还用荧光指示剂染料检测缺氧暴露期间大鼠海马细胞内游离钙浓度（[Ca2+](i)）的升高。这些结果表明，FOP显微镜系统足以适用于利用荧光技术研究组织结构和生理活动的体内实验，甚至是在深层区域。
The feasibility of a fiber-optic plate (FOP) microscope system employing a bundle of optical fibers and videomicroscopy for in vivo experiments was investigated. The FOP used here consisted of optical fibers 3 mu m in diameter. By inserting the FOP into an animal, optical signals from the deep-lying tissue invisible from the surface could be obtained as two-dimensional images. Using this system, hippocampal cells stained with a fluorescent dye in an anesthetized rat were visualized. Elevation of intracellular free calcium concentration ([Ca2+](i)) in the hippocampus of the rat during anoxic exposure was also detected with a fluorescent indicator dye. These results showed that the FOP microscope system was sufficiently applicable to in vivo experiments for studying tissue structure and physiological activity even in the deep regions with fluorometric techniques.