Meiotic failure in male mice lacking an X-linked factor
Meiotic failure in male mice lacking an X-linked factor
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DOI：
10.1101/gad.1613608
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发表时间：
2008-03-01
影响因子：
10.5
通讯作者：
Wang, Peijing Jeremy
中科院分区：
文献类型：
--
作者：
Yang, Fang;Gell, Katarina;Wang, Peijing Jeremy
性染色体的减数分裂沉默可能导致它们在进化过程中减数分裂特异基因的耗尽。在这里，我们通过报告TEX11被鉴定为第一个X编码的小鼠减数分裂特异因子来挑战这一假设。TEX11在减数分裂染色体的突触区域形成离散的焦点，似乎是参与重组的减数分裂结节的新成分。TEX11功能的丧失导致染色体不联会和交叉形成减少，导致精母细胞分别在粗线期和后期I期消失。具体地说，带有非突触常染色体的TEX11缺陷精母细胞在粗线期发生凋亡，而那些只有非突触的性染色体的精母细胞则进展。然而，在粗线期存活的细胞在第一次减数分裂时表现出染色体不分离，导致细胞死亡和雄性不育。TEX11与SYCP2相互作用，SYCP2是联会复合体横向元件的组成部分。因此，TEX11促进突触的启动和/或维持以及交叉的形成，并可能在这两个减数分裂过程之间提供物理联系。
Meiotic silencing of sex chromosomes may cause their depletion of meiosis-specific genes during evolution. Here, we challenge this hypothesis by reporting the identification of TEX11 as the first X-encoded meiosis-specific factor in mice. TEX11 forms discrete foci on synapsed regions of meiotic chromosomes and appears to be a novel constituent of meiotic nodules involved in recombination. Loss of TEX11 function causes chromosomal asynapsis and reduced crossover formation, leading to elimination of spermatocytes, respectively, at the pachytene and anaphase I stages. Specifically, TEX11-deficient spermatocytes with asynapsed autosomes undergo apoptosis at the pachytene stage, while those with only asynapsed sex chromosomes progress. However, cells that survive the pachytene stage display chromosome nondisjunction at the first meiotic division, resulting in cell death and male infertility. TEX11 interacts with SYCP2, which is an integral component of the synaptonemal complex lateral elements. Thus, TEX11 promotes initiation and/or maintenance of synapsis and formation of crossovers, and may provide a physical link between these two meiotic processes.