Selection of RNA aptamers against human influenza virus hemagglutinin using surface plasmon resonance
Selection of RNA aptamers against human influenza virus hemagglutinin using surface plasmon resonance
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DOI：
10.1016/j.ab.2005.04.013
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发表时间：
2005-07-15
影响因子：
2.9
通讯作者：
Kumar, PKR
中科院分区：
文献类型：
--
作者：
Misono, TS;Kumar, PKR
适配体是对其同源配体具有高亲和力和特异性的功能性核酸，通过反复的选择和扩增从核酸库中分离出来。在目前的研究中…我们使用表面等离子体共振（Biacore）作为一种有效的方法来选择与人流感病毒血凝素（HA）结合的适体。这一过程使我们能够同时特异性地监测和选择目标结合的适体。这些研究不仅获得了与流感病毒HA高亲和力结合的适体，而且揭示了HA识别的一致序列5'-GUCGNCNU(N)(2-3)GUA-3。(c) 2005爱思唯尔公司版权所有。
Aptamers are functional nucleic acids possessing high affinity and specificity to their cognate ligands and are isolated from a library of nucleic acids by iterative rounds of selection and amplification. In the current study.. we used surface plasmon resonance (Biacore) as an efficient methodology for selecting aptamers that bind to hemagglutinin (HA) of human influenza virus. This procedure allowed us to monitor and select the target-bound aptamers specifically and simultaneously. These studies not only yielded an aptamer that binds to the HA of influenza virus with high affinity but also revealed the consensus sequence, 5'-GUCGNCNU(N)(2-3)GUA-3, for HA recognition. (c) 2005 Elsevier Inc. All rights reserved.