The formation of vault-tubes: a dynamic interaction between vaults and vault PARP
The formation of vault-tubes: a dynamic interaction between vaults and vault PARP
复制标题
拱顶管的形成：拱顶和拱顶 PARP 之间的动态相互作用
DOI：
10.1242/jcs.00749
复制
发表时间：
2003
影响因子：
4
通讯作者：
E. Wiemer
中科院分区：
文献类型：
--
作者：
A. van Zon;Marieke H. Mossink;M. Schoester;A. Houtsmuller;G. Scheffer;R. Scheper;P. Sonneveld;E. Wiemer
穹窿是由一个主要的穹窿蛋白（MVP），两个次要的穹窿蛋白[端粒酶相关蛋白1（TEP 1），穹窿聚（ADP-核糖）聚合酶（VPARP）]和小的非翻译RNA分子组成的桶形细胞质核糖核蛋白颗粒。并非所有在细胞中表达的TEP 1和VPARP都与穹窿体结合。已知TEP 1与端粒酶复合物相关，而VPARP也存在于核基质和细胞质簇（VPARP-杆）中。我们研究了非小细胞肺癌细胞系中表达MVP标记的绿色荧光蛋白的拱顶复合物的亚细胞定位和动力学。使用定量荧光恢复后光漂白（FRAP），它表明，拱顶移动温度独立的扩散。然而，在室温（21°C）下孵育导致在细胞质中形成不同的管状结构。提高温度可以逆转这一过程。当拱顶管形成时，细胞质中存在较少或没有VPARP-杆，表明VPARP掺入拱顶管中。MVP分子必须通过其卷曲螺旋结构域彼此相互作用以形成拱顶管。此外，微管的稳定性影响了21°C下拱顶管形成的效率。使用共聚焦显微镜检查管的动力学和结构。我们的数据表明，一个直接的和动态的关系，拱顶和VPARP，提供进一步的线索，解开拱顶的功能。
Vaults are barrel-shaped cytoplasmic ribonucleoprotein particles that are composed of a major vault protein (MVP), two minor vault proteins [telomerase-associated protein 1 (TEP1), vault poly(ADP-ribose) polymerase (VPARP)] and small untranslated RNA molecules. Not all expressed TEP1 and VPARP in cells is bound to vaults. TEP1 is known to associate with the telomerase complex, whereas VPARP is also present in the nuclear matrix and in cytoplasmic clusters (VPARP-rods). We examined the subcellular localization and the dynamics of the vault complex in a non-small cell lung cancer cell line expressing MVP tagged with green fluorescent protein. Using quantitative fluorescence recovery after photobleaching (FRAP) it was shown that vaults move temperature independently by diffusion. However, incubation at room temperature (21°C) resulted in the formation of distinct tube-like structures in the cytoplasm. Raising the temperature could reverse this process. When the vault-tubes were formed, there were fewer or no VPARP-rods present in the cytoplasm, suggesting an incorporation of the VPARP into the vault-tubes. MVP molecules have to interact with each other via their coiled-coil domain in order to form vault-tubes. Furthermore, the stability of microtubules influenced the efficiency of vault-tube formation at 21°C. The dynamics and structure of the tubes were examined using confocal microscopy. Our data indicate a direct and dynamic relationship between vaults and VPARP, providing further clues to unravel the function of vaults.
Mr 193,000 穹窿蛋白在多重耐药癌细胞系中表达上调。
DOI：
--
发表时间：
2000
期刊：
Cancer research.
影响因子：
--
作者：
Schroeijers,AB;Siva,AC;Scheffer,GL;deJong,MC;Bolick,SC;Dukers,DF;Slootstra,JW;Meloen,RH;Wiemer,E;Kickhoefer,VA;Rome,LH;Scheper,RJ
通讯作者：
Scheper,RJ
穹窿核糖核蛋白颗粒定位于核孔复合体的证据。
影响因子：
4
作者：
Chugani,DC;Rome,LH;Kedersha,NL
通讯作者：
Kedersha,NL