[Immunoglobulin G subclass distribution of anti-intercellular substance antibodies in pemphigus].
[Immunoglobulin G subclass distribution of anti-intercellular substance antibodies in pemphigus].
复制标题
天疱疮抗细胞间质抗体免疫球蛋白G亚类分布[J].
DOI：
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发表时间：
1995
影响因子：
0.9
通讯作者：
J. Bazex
中科院分区：
文献类型：
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作者：
C. Trémeau;F. Oksman;J. Bazex
介绍
本研究的目的是分析天疱疮抗上皮细胞表面（ECS）抗体的IgG亚类分布，并确定其是否根据临床特征而有所不同。
材料和方法
用鼠抗人IgG亚类单克隆抗体对27例天疱疮患者的25份皮肤活检标本和16份血清标本进行直接和间接IF染色。
结果
在25例活检中，21例观察到IgG1沉积，2例观察到IgG2沉积，0例观察到IgG3沉积，25例观察到IgG4沉积。在所有16份血清中检测到IgG1抗ECS抗ECS抗体，IgG2 1份，IgG3 1份，IgG4 15份。根据研究的临床参数，抗ECS IgG亚类分布无差异。
讨论
本研究中观察到的IgG1和IgG4亚类的同种型限制与先前报告的结果相似。研究样本的异质性分布和数量较少，无法证明与临床参数的相关性。
INTRODUCTION
The purpose of this study was to analyze the IgG subclass distribution of pemphigus anti-epithelial cell surface (ECS) antibodies and to determine whether it differs according to clinical features.
MATERIALS AND METHODS
25 skin biopsies and 16 serum samples, obtained from 27 cases of pemphigus, were analyzed by direct and indirect IF staining, with mice anti-human IgG subclasses monoclonal antibodies.
RESULTS
IgG1 deposits were observed in 21 of 25, IgG2 in 2, IgG3 in 0, and IgG4 in the 25 biopsies. IgG1 anti-ECS anti-ECS antibodies were detected in all 16 sera, IgG2 in 1, IgG3 in 1, and IgG4 in 15 sera. The anti-ECS IgG subclass distribution does not differ according to the clinical parameters studied.
DISCUSSION
The isotypic restriction to IgG1 and IgG4 subclasses, observed in this study, is similar to previously reported results. The heterogenous distribution and the small number of the studied samples did not allow to put in evidence a correlation with the clinical parameters.