QUANTITATION OF NEUTROPHIL MIGRATION IN ACUTE BACTERIAL PNEUMONIA IN RABBITS
QUANTITATION OF NEUTROPHIL MIGRATION IN ACUTE BACTERIAL PNEUMONIA IN RABBITS
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DOI：
10.1152/jappl.1994.77.6.2593
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发表时间：
1994-12-01
影响因子：
3.3
通讯作者：
HOGG, JC
中科院分区：
文献类型：
--
作者：
DOERSCHUK, CM;MARKOS, J;HOGG, JC
循环中的中性粒细胞必须减慢速度，粘附在血管壁上，并从微血管系统中迁移到组织和空气空间中，以保护肺免受微生物的侵害。本研究旨在提供有关这些步骤的定量信息。将肺炎链球菌滴注到新西兰白色兔的左肺下叶以诱导肺炎，并将该叶与对侧肺的相同区域进行比较。血流量的分布通过使用放射性标记的大聚集白蛋白来确定，并且使用单星蓝定量毛细血管床内的灌注模式。通过将循环中性粒细胞计数与血流量相乘，确定输送至肺炎部位的中性粒细胞数量。结果表明，Cr-51标记的中性粒细胞的保留增加，在肺部区域2，4和8小时后滴注的生物体。在所有时间点的肺炎区域以及1和4 h的对照区域中，毛细血管内中性粒细胞数量增加。中性粒细胞迁移发生在肺炎部位，但输送到该区域的总中性粒细胞中只有1-2%迁移出肺血管进入空气间隙。我们的结论是，循环中性粒细胞经历了一个普遍的反应，增加了他们的边缘在整个肺，增加边缘在肺炎网站的改变毛细血管流量的分布，和大多数的中性粒细胞交付到一个肺炎网站返回到循环没有迁移到空气空间。
The circulating neutrophils must slow down, adhere to the vessel walls, and migrate out of the microvasculature into the tissue and air spaces to defend the lung against microorganisms. The present study was designed to provide quantitative information about each of these steps. Streptococcus pneumoniae was instilled into the left lower lobe of New Zealand White rabbits to induce a pneumonia, and this lobe was compared with the same region of the opposite lung. The distribution of blood flow was determined by using radiolabeled macroaggregated albumin, and the patterns of perfusion within the capillary bed were quantitated using Monastral blue. The number of neutrophils delivered to the pneumonic site was determined by multiplying the circulating neutrophil count and blood flow. The results show that retention of Cr-51-labeled neutrophils was increased in the pneumonic region 2, 4, and 8 h after instillation of the organisms. The number of intracapillary neutrophils was increased in the pneumonic regions at all time points and in the control regions at 1 and 4 h. Neutrophil migration occurred in the pneumonic site, but only 1-2% of the total neutrophils delivered to the region migrated out of the pulmonary vessels into the air space. We conclude that the circulating neutrophils undergo a generalized response that increases their margination throughout the lung, that increased margination in the pneumonic site changes the distribution of capillary flow, and that the majority of neutrophils delivered to a pneumonic site are returned to the circulation without migrating into the air space.