Eyeless/Pax6 initiates eye formation non-autonomously from the peripodial epithelium
Eyeless/Pax6 initiates eye formation non-autonomously from the peripodial epithelium
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DOI：
10.1242/dev.163329
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发表时间：
2018-08-01
期刊：
影响因子：
4.6
通讯作者：
Kumar, Justin P.
中科院分区：
文献类型：
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作者：
Baker, Luke R.;Weasner, Bonnie M.;Kumar, Justin P.
转录因子Pax6被认为是眼睛形成的主控基因，因为(1)它存在于所有有视觉系统的动物的基因组和视网膜/晶状体中；(2)它伴随着严重的视网膜缺陷而丧失；(3)Pax6基因能够在整个动物界相互替代；(4)Pax6基因能够在果蝇和哺乳动物中诱导异位眼/晶状体。Pax6的许多作用首次在果蝇中被阐明，是通过对失眼基因(Ey)的研究，该基因控制着整个眼睛触角成像盘的生长和眼睛的命运指定。我们发现，EY在控制花纹形成的过程中，在盆周上皮细胞中也扮演着令人惊讶的角色。它调节十叉神经麻痹(DPP)的表达，这是启动眼睛本身的形态发生沟所必需的。眼球周围上皮内EY的丢失会导致DPP在眼内表达的丧失，皱纹的启动失败，以及视网膜发育的丧失。这些发现揭示了Pax6如何控制果蝇眼睛发育的一种意想不到的机制。
The transcription factor Pax6 is considered the master control gene for eye formation because (1) it is present within the genomes and retina/lens of all animals with a visual system; (2) severe retinal defects accompany its loss; (3) Pax6 genes have the ability to substitute for one another across the animal kingdom; and (4) Pax6 genes are capable of inducing ectopic eye/lens in flies and mammals. Many roles of Pax6 were first elucidated in Drosophila through studies of the gene eyeless (ey), which controls both growth of the entire eye-antennal imaginal disc and fate specification of the eye. We show that Ey also plays a surprising role within cells of the peripodial epithelium to control pattern formation. It regulates the expression of decapentaplegic (dpp), which is required for initiation of the morphogenetic furrow in the eye itself. Loss of Ey within the peripodial epithelium leads to the loss of dpp expression within the eye, failure of the furrow to initiate, and abrogation of retinal development. These findings reveal an unexpected mechanism for how Pax6 controls eye development in Drosophila.