Characterization of novel Omp31 antigenic epitopes of Brucella melitensis by monoclonal antibodies.
Characterization of novel Omp31 antigenic epitopes of Brucella melitensis by monoclonal antibodies.
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单克隆抗体鉴定羊布鲁氏菌新 Omp31 抗原表位
DOI：
10.1186/s12866-017-1025-3
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发表时间：
2017-05-15
期刊：
影响因子：
4.2
通讯作者：
Li C
中科院分区：
文献类型：
--
作者：
Li J;Hu F;Chen S;Luo P;He Z;Wang W;Allain JP;Li C
研究背景布鲁氏菌病是一种严重的人畜共患病。布鲁氏菌的检测和鉴定对于预防或治疗人类和动物的布鲁氏菌病至关重要。外膜蛋白31（Omp31）是布鲁氏菌除B外的主要蛋白质。结果共制备了22株抗布鲁氏菌Omp31单克隆抗体（mAb）。Melitensis的13个抗原表位分别识别5个线性表位，7个半构象表位，2个构象表位。单克隆抗体的同种型为11种（50%）IgG2a、5种（23%）IgG1和6种（27%）IgM。基于表位识别和反应性水平，包括3个IgM和5个IgG克隆的8个mAb被认为是高反应性和潜在的诊断抗体。其中7A3（IgG1）、5B1（IgG2a）、2C1（IgG2a）和5B3（IgG2a）与B的不同保守线性表位反应。melitensis，B. ovis，B. suis和B.当用几种免疫测定法测试时，5H3（IgG2a）与Omp 31的构象表位高度反应。
BackgroundBrucellosis is a severe zoonotic disease worldwide. Detection and identification of Brucella species are essential to prevent or treat brucellosis in humans and animals. The outer membrane protein-31 (Omp31) is a major protein of Brucellae except for B. abortus, while the Omp31 antigenic epitopes have not been extensively characterized yet.ResultsA total of 22 monoclonal antibodies (mAbs) were produced against Omp31 of Brucella (B.) melitensis, of which 13 recognized five linear epitopes, 7 reacted with semi-conformational epitopes and 2 reacted with conformational epitopes, respectively. The mAb isotypes were 11 (50%) IgG2a, 5 (23%) IgG1 and 6 (27%) IgM. On the basis of epitope recognition and reactivity levels, 8 mAbs including 3 IgM and 5 IgG clones were considered as highly reactive and potentially diagnostic antibodies. Among these mAbs, 7A3 (IgG1), 5B1 (IgG2a), 2C1 (IgG2a) and 5B3 (IgG2a) reacted with differently conserved linear epitopes of B. melitensis, B. ovis, B. suis and B. canis strains, while 5H3 (IgG2a) highly reacted with a conformational epitope of Omp31 when tested with several immunoassays.Conclusions
影响因子：
3.7
作者：
Zhang L;Yin S;Tan W;Xiao D;Weng Y;Wang W;Li T;Shi J;Shuai L;Li H;Zhou J;Allain JP;Li C
通讯作者：
Li C