NEUROFIBRILLARY TANGLES OF ALZHEIMER-DISEASE SHARE ANTIGENIC DETERMINANTS WITH THE AXONAL MICROTUBULE-ASSOCIATED PROTEIN TAU (TAU)
NEUROFIBRILLARY TANGLES OF ALZHEIMER-DISEASE SHARE ANTIGENIC DETERMINANTS WITH THE AXONAL MICROTUBULE-ASSOCIATED PROTEIN TAU (TAU)
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DOI：
10.1073/pnas.83.11.4040
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发表时间：
1986-06-01
影响因子：
11.1
通讯作者：
BINDER, LI
中科院分区：
文献类型：
--
作者：
WOOD, JG;MIRRA, SS;BINDER, LI
在阿尔茨海默病和老年大脑中发现的神经原纤维缠结与正常或异常的细胞骨架蛋白之间的关系仍然难以捉摸。尽管免疫组织化学研究得出了不同的结果，但大多数定位于神经原纤维缠结的抗原决定因子是通常存在于神经元核周或树突中的细胞骨架成分。我们报道了微管相关蛋白tau （.tau）单克隆抗体对神经原纤维缠结的光镜和电镜免疫标记。组织切片的去磷酸化不仅增加了雌性的数量。-阳性缠结，但也产生明显的神经斑块阳性免疫反应。.tau.的本地化。一种轴突蛋白，特别是核周围的神经原纤维缠结表明。tau蛋白的异常合成、修饰或聚集。可能诱发异常的细胞骨架-细胞器相互作用，随后干扰轴突流动，并最终形成缠结。
The relationship of the neurofibrillary tangle, found in Alzheimer disease and aged brains, to normal or abnormal cytoskeletal proteins remains elusive. Although immunohistochemical studies have yielded disparate results, most antigenic determinants localized to neurofibrillary tangles are cytoskeletal constituents normally present in neuronal perikarya or dendrites. We report light and electron microscopic immunolabeling of neurofibrillary tangles by a monoclonal antibody to the microtubule-associated protein tau (.tau.). Dephosphorylation of tissue slices not only increased the number of .female.-positive tangles but also produced marked positive immunoreactivity of neuritic plaques. The localization of .tau., an axonal protein, to neurofibrillary tangles in the perikaryon in particular suggests that abnormal synthesis, modification, or aggregation of .tau. may induce aberrant cytoskeletal-cell organelle interactions, subsequent interference with axonal flow, and resultant tangle formation.