Trimeric G protein-dependent frizzled signaling in Drosophila
Trimeric G protein-dependent frizzled signaling in Drosophila
复制标题
DOI：
10.1016/j.cell.2004.11.014
复制
发表时间：
2005-01-14
期刊：
影响因子：
64.5
通讯作者：
Tomlinson, A
中科院分区：
文献类型：
--
作者：
Katanaev, VL;Ponzielli, R;Tomlinson, A
卷曲蛋白（Fz）是发育过程中抑制关键细胞信号的蛇形受体。丝氨酸蛋白酶受体通常通过相关的三聚体G蛋白复合物向下游效应物发出信号。然而，迄今为止，缺乏明确的证据，三聚体G蛋白复合物的Fz家族的受体的作用。在这里，我们展示了Go亚基（Go）在介导果蝇中Fz受体转导的两种不同途径中的作用：Writ和平面极性途径。Go是这两种途径转导所必需的，上位性实验表明它是Fz的直接转导子。虽然野生型形式的过表达效应是受体依赖性的，但激活形式（Go-GTP）可以在受体被去除时发出信号。因此，Go可能是三聚体G蛋白复合物的一部分，其直接将Fz信号从膜转导到下游组分。
Frizzled (Fz) proteins are serpentine receptors that transduce critical cellular signals during development. Serpentine receptors usually signal to downstream effectors through an associated trimeric G protein complex. However, clear evidence for the role of trimeric G protein complexes for the Fz family of receptors has hitherto been lacking. Here, we show roles for the Go, subunit (Go) in mediating the two distinct pathways transduced by Fz receptors in Drosophila: the Writ and planar polarity pathways. Go is required for transduction of both pathways, and epistasis experiments suggest that it is an immediate transducer of Fz. While overexpression effects of the wild-type form are receptor dependent, the activated form (Go-GTP) can signal when the receptor is removed. Thus, Go is likely part of a trimeric G protein complex that directly tranduces Fz signals from the membrane to downstream components.