Lymphatic Network and Lymphangiogenesis in the Gastric Wall
Lymphatic Network and Lymphangiogenesis in the Gastric Wall
复制标题
胃壁的淋巴网络和淋巴管生成
DOI：
10.1177/002215540305100308
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发表时间：
2003
影响因子：
3.2
通讯作者：
S. Kato
中科院分区：
文献类型：
--
作者：
R. Ji;S. Kato
内皮细胞高度特异性的生长因子家族在10多年前被发现，其中血管内皮生长因子C受体（VEGFR-3）参与淋巴发育和再生的调节。对成人和胎儿胃壁淋巴网络和淋巴管生成的比较研究主要采用5′-核苷酸酶（5′-Nase）和VEGFR-3联合免疫组化方法。在全挂载制备中，发育中的淋巴网络比成熟的淋巴网络具有更少的盲端和分支。许多圆形淋巴样结构在胚胎早期表达VEGFR-3, 5 ' -Nase活性较弱，显示出明显的淋巴内皮形态学特征。这些新形成的淋巴管在粘膜下层和浆膜中有明显的积聚，并且在不同样本中VEGFR-3与内皮细胞结合的强度不同。在内皮细胞的管腔表面以及一些细胞器和管腔内淋巴细胞的膜上发现了抗vegfr -3的反应产物。这些发现表明，聚集性发育淋巴的活跃增殖特征可能为其发芽和生长创造了有利的环境，这可能是该地区淋巴引流的功能要求。
A family of growth factors highly specific for endothelial cells was identified more than 10 years ago, in which the receptor of vascular endothelial growth factor C (VEGFR-3) is implicated in the regulation of lymphatic development and regeneration. Comparative studies on the lymphatic network and lymphangiogenesis have been done mainly using combined 5′-nucleotidase (5′-Nase) enzyme and VEGFR-3 immunohistochemical approaches in adult and fetal gastric walls. Developing lymphatic networks represented fewer blind ends and branches than mature networks in whole-mount preparations. Many circular lymphatic-like structures exhibited VEGFR-3 expression and weak 5′-Nase activity in the early embryonic stage, showing visible morphological properties in the lymphatic endothelium. These newly formed lymphatics showed an obvious accumulation in the submucosa and serosa and a variation in the intensity of VEGFR-3 binding to endothelial cells among samples. A reaction product for anti-VEGFR-3 was found on the luminal surface of endothelial cells and on the membrane of some organelles and intraluminal lymphocytes. These findings indicate that an active proliferating feature of the clustered developing lymphatics may create a favorable environment for their sprouting and growth, which may serve as a functional requirement for lymph drainage in the region.