West Nile virus in Southern Portugal, 2004
West Nile virus in Southern Portugal, 2004
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DOI：
10.1089/vbz.2005.5.410
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发表时间：
2005-12-01
影响因子：
2.1
通讯作者：
Novo, MT
中科院分区：
文献类型：
--
作者：
Esteves, A;Almeida, APG;Novo, MT
2004年夏季，通过逆转录聚合酶链反应（RT-PCR）在葡萄牙南部收集的57个蚊子池中的6个中检测到西尼罗病毒（WNV）基因组RNA，感染率（IR）为2.8/ 1000只蚊子。从未饲养的淡色库蚊和univitatus库蚊雌性中扩增出的NS5编码区217个核苷酸片段的系统发育分析表明，它们与地中海盆地流行的西尼罗河病毒株有密切关系（意大利，1998年；法国，2000年；摩洛哥，2003年）。本简短报告中的数据表明，西尼罗河病毒在葡萄牙南部的蚊子中存在，需要对该国境内的病毒活动进行长期监测。
West Nile virus (WNV) genomic RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in six out of 57 mosquito pools collected in Southern Portugal, during the summer of 2004, yielding an infection rate (IR) of 2.8/1,000 mosquitoes. Phylogenetic analysis of a 217-nucleotide fragment of the NS5 coding region, amplified from Culex pipiens s.l. and Culex univittatus unfed females, demonstrated a close relationship with WNV strains circulating in the Mediterranean basin (Italy, 1998; France, 2000; Morocco, 2003). The data in this short report demonstrate the presence of WNV in mosquitoes in Southern Portugal and the need of permanent surveillance for viral activity within the country.