In situ modification of chromatography adsorbents using cold atmospheric pressure plasmas
In situ modification of chromatography adsorbents using cold atmospheric pressure plasmas
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DOI：
10.1063/1.4807391
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发表时间：
2013-05
影响因子：
4
通讯作者：
P. Olszewski;Thomas C. Willett;E. Theodosiou;O. Thomas;J. Walsh
中科院分区：
文献类型：
--
作者：
P. Olszewski;Thomas C. Willett;E. Theodosiou;O. Thomas;J. Walsh
高效生产日益复杂的生物制药需要开发具有两层或多层的新型色谱材料，每层提供不同的功能。这封信报道了原位改性的商业珠状阴离子交换吸附剂使用大气压等离子体产生的气泡内。结果表明，以这种方式暴露于He-O2等离子体产生质粒DNA与吸附剂外部的表面结合的显著减少，核心蛋白结合能力的损失最小;因此，产生了在同一珠内表现出尺寸排除和阴离子交换功能的双层层析材料。
Efficient manufacturing of increasingly sophisticated biopharmaceuticals requires the development of new breeds of chromatographic materials featuring two or more layers, with each layer affording different functions. This letter reports the in situ modification of a commercial beaded anion exchange adsorbent using atmospheric pressure plasma generated within gas bubbles. The results show that exposure to He-O2 plasma in this way yields significant reductions in the surface binding of plasmid DNA to the adsorbent exterior, with minimal loss of core protein binding capacity; thus, a bi-layered chromatography material exhibiting both size excluding and anion exchange functionalities within the same bead is produced.