Epidemiological study of kala-azar by direct agglutination test in two rural communities of eastern Nepal
Epidemiological study of kala-azar by direct agglutination test in two rural communities of eastern Nepal
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DOI：
10.1111/j.1365-3156.2004.01212.x
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发表时间：
2004-04-01
影响因子：
3.3
通讯作者：
Karki, BMS
中科院分区：
文献类型：
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作者：
Koirala, S;Karki, P;Karki, BMS
我们进行了黑热病的血清表皮学研究，在两个地方性社区（Kasaini和Gidhaniya）位于尼泊尔东部的Terai（平原）。采用直接凝集试验（DAT）进行血清学筛查。毛细血管血液样本收集滤纸法从601（96%）人的总人口628 Kasaini和482（94%）人的515 Gidhaniya。1083份血清中DAT阳性66份（6.09%），阳性率1：2000。男女血清流行率之比为1.44：1，15岁及以上年龄组受影响最大。在66例DAT阳性病例的骨髓穿刺标本中，仅19例为杜氏利什曼原虫阳性（LD体）。在47例DAT阳性而LD阴性的病例中，有3例为黑热病临床活动性病例。另外9例在6个月随访期间出现黑热病临床症状，23例为既往接受过黑热病治疗（1年内）的病例。这项研究的结果表明，在滤纸上的DAT作为黑热病的监测在社区一级的筛选试验的潜力。
We conducted a sero-epidermological study of kala-azar in two endemic communities (Kasaini and Gidhaniya) situated in the Terai (plain) of eastern Nepal. Direct agglutination test (DAT) was used as a serological test for screening. Capillary blood samples were collected by filter paper method from 601 (96%) people of a total population of 628 in Kasaini and from 482 (94%) people of 515 in Gidhaniya. Positive DAT titres (1:2000) were found in 66 (6.09%) of 1083 sera tested. The male-female sero-prevalence ratio was 1.44:1 and the age group of 15 years and above was most affected. Among the bone marrow aspirates collected from 66 DAT seropositive cases, only 19 were positive for Leishmania donovani (LD bodies). Of the 47 DAT seropositive but LD bodies' negative cases, three were clinically active cases of kala-azar. Another nine developed clinical symptoms of kala-azar during 6 months follow-up and 23 were cases that had received prior treatment for kala-azar (within 1 year). The results of this study show the potential of the DAT on filter paper as a screening test for the surveillance of kala-azar at a community level.