Growth of Lucilia cuprina larvae following treatment of sheep divergently selected for fleece rot and fly strike with monoclonal antibodies to T lymphocyte subsets and interferon gamma
Growth of Lucilia cuprina larvae following treatment of sheep divergently selected for fleece rot and fly strike with monoclonal antibodies to T lymphocyte subsets and interferon gamma
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DOI：
10.1016/0020-7519(96)00048-3
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发表时间：
1996-07-01
影响因子：
4
通讯作者：
Husband, AJ
中科院分区：
文献类型：
--
作者：
Colditz, IG;Eisemann, CH;Husband, AJ
首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容用抗T淋巴细胞亚群和干扰素γ的单抗处理不同选择的绵羊后，紫光绿蝇幼虫的生长。《国际寄生虫学杂志》26：775-782。在由斑潜蝇幼虫引起的皮肤蝇丝虫病期间，真皮下有大量的淋巴细胞渗入。为确定该浸液对幼虫生长的影响，在绵羊幼虫实验感染前和感染过程中，分别经静脉注射抗CD_4、CD_8或WC_1淋巴细胞亚群决定簇的单抗。同时，还观察了静脉注射单抗对绵羊干扰素-γ的影响。这些实验是在18个月大的处女母羊身上进行的，这些母羊对细菌性皮炎/皮肤蝇虫病具有遗传抵抗力或易感性。单抗诱导幼虫生长部位的血液和皮肤中CD8+和Wc1+亚群的严重耗竭。从单抗到CD4+，血液或皮肤中的淋巴细胞只有适度的减少。在感染后20或50小时，单抗处理不影响幼虫的生长或存活。抗性和感病两种基因型的幼虫生长速度没有差异。在感染的前50小时内，没有发现T淋巴细胞亚群或细胞因子干扰素-γ在调节幼虫生长方面的作用。似乎不太可能是T淋巴细胞依赖的免疫效应机制有助于在田间抗性基因中看到较低的苍蝇袭击流行率。版权所有(C)1996澳大利亚寄生虫学学会。
Colditz I. G., Eisemann C. H., Tellam R. L., McClure S. J., Mortimer S. I. & Husband A, J. 1996. Growth of Lucilia cuprina larvae following treatment of sheep divergently selected for fleece rot and fly strike with monoclonal antibodies to T lymphocyte subsets and interferon gamma. International Journal for Parasitology 26: 775-782. Intensive lymphocytic infiltration of the underlying dermis occurs during cutaneous myiasis caused by larvae of the blow fly, Lucilia cuprina. To determine the effect of this infiltrate on larval growth, monoclonal antibodies (mAb) to CD4, CD8 or WC1 lymphocyte subset determinants were injected intravenously before and during experimental infection of sheep with larvae. The effect of intravenous injection of mAb to ovine interferon (IFN) gamma was also examined. The experiments were performed in 18-month-old maiden ewes with genetic resistance or susceptibility to the disease complex, bacterial dermatitis/cutaneous myiasis. mAbs induced profound depletion of CD8+ and WC1+ subpopulations from blood and skin at sites of larval growth. mAb to CD4+ gave only a moderate reduction in lymphocytes from blood or skin. mAb treatments did not modify larval growth or survival at 20 or 50 h after infection. Larval growth rates did not differ between resistant and susceptible genotypes. No evidence was found for a role of T lymphocyte subpopulations or the cytokine IFN gamma in modifying larval growth during the first 50 h of infection. It seems unlikely that T lymphocyte-dependent immunological effector mechanisms contribute to the lower prevalence of fly strike seen in the resistant genotype in the field. Copyright (C) 1996 Australian Society for Parasitology.