Decreased [3H]serotonin and [3H]spiperone binding consequent to lipid peroxidation in rat cortical membranes.
Decreased [3H]serotonin and [3H]spiperone binding consequent to lipid peroxidation in rat cortical membranes.
复制标题
大鼠皮质膜脂质过氧化导致[3H]血清素和[3H]螺哌隆结合减少。
DOI：
10.1016/0006-291x(82)91349-3
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发表时间：
1982
影响因子：
3.1
通讯作者：
Hochstein,P
中科院分区：
文献类型：
--
作者：
Muakkassah-Kelly,SF;Andresen,JW;Shih,JC;Hochstein,P
研究了脂质过氧化对两类血清素结合位点的影响。大鼠皮质膜与抗坏血酸依赖的过氧化系统孵育导致丙二醛的形成和[3H] 5 -羟色胺和[3H]spiperone在处理膜上的特异性结合显著降低。当抗坏血酸浓度从0.025到6.0 mM变化时，丙二醛产量在0.5 mM抗坏血酸时增加到最大值，然后下降。相反，[3H]血清素和[3H]spiperone的特异性结合在0.5 mM抗坏血酸时降至最低，然后增加。数据的回归分析显示，这两个结合位点的减少与脂质过氧化呈线性相关。
The effect of lipid peroxidation on two types of serotonin binding sites was investigated. Incubation of rat cortical membranes with an ascobate-dependent peroxidizing system resulted in the formation of malonyldialdehyde and significant decreases in the specific binding of [3H]serotonin and [3H]spiperone to the treated membranes. When ascorbate concentrations were varied from 0.025 to 6.0 mM, malonyldialdehyde production increased to a maximum at 0.5 mM ascorbate and then declined. Conversely, the specific binding of [3H]serotonin and [3H]spiperone decreased to a minimum at 0.5 mM ascorbate and then increased. Regression analysis of the data revealed that the decrease in the two binding sites was linearly correlated to lipid peroxidation.