Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques
Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques
复制标题
通过培养依赖和非培养技术测定草鱼（Ctenopharyngodon idellus）（Valenciennes）肠粘膜中原生细菌群落的多样性
DOI：
10.1111/are.12391
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发表时间：
2015-10-01
影响因子：
2
通讯作者：
Wang, Guitang
中科院分区：
文献类型：
--
作者：
Li, Huan;Zhong, Qiuping;Wang, Guitang
采用传统培养技术和16 S rDNA序列测定方法对草鱼（Ctenopharyngodon idellus）胃粘膜相关的土著微生物群进行了研究。从可培养菌群中检测到21种致病菌，其中气单胞菌属、希瓦氏菌属、乳球菌属、沙雷氏菌属、短芽孢杆菌属、代尔夫特氏菌属、假单胞菌属、泛菌属、肠杆菌属、布丘氏菌属和耶尔森氏菌属是它们的近亲。直接从大肠杆菌肠粘膜中提取基因组DNA。idellus，从6个克隆文库中随机获得609个细菌克隆，从1个文库中随机获得99个古细菌克隆，分为67个细菌和4个古细菌类群。序列分析表明，肠粘膜中存在多种细菌，其中变形菌门、厚壁菌门和拟杆菌门占优势，其次是放线菌门、疣微菌门和异常球菌-栖热菌门。不同水环境鱼类肠道粘膜中的土著细菌群落结构不尽相同（C-s < 0.80），但γ-变形菌门是一个常见的细菌类群。与细菌群落相比，从一个文库中获得的古菌群落由泉古菌和广古菌组成。这些结果表明，分子方法有利于培养独立的研究，鱼肠道粘膜窝藏比以前认识到更大的细菌多样性。草鱼肠道栖息地选择特定的细菌类群，尽管在宿主环境的显着差异。
Traditional culture-based technique and 16S rDNA sequencing method were used to investigate the mucosa-associated autochthonous microbiota of grass carp (Ctenopharyngodon idellus). Twenty-one phylotypes were detected from culturable microbiota, with Aeromonas, Shewanella, Lactococcus, Serratia, Brevibacillus, Delftia, Pseudomonas, Pantoea, Enterobacter, Buttiauxella and Yersinia as their closest relatives. Genomic DNA was directly extracted from the gut mucosa of C. idellus originating from six different geographical regions, and used to generate 609 random bacterial clones from six clone libraries and 99 archaeal clones from one library, which were grouped into 67 bacterial and four archaeal phylotypes. Sequence analysis revealed that the intestinal mucosa harboured a diversified bacterial microbiota, where Proteobacteria, Firmicutes and Bacteroidetes were dominant, followed by Actinobacteria, Verrucomicrobia and Deinococcus-Thermus. The autochthonous bacterial communities in the gut mucosa of fish from different aquatic environments were not similar (C-s < 0.80), but gamma-Proteobacteria was a common bacterial class. In comparison to bacterial communities, the archaeal community obtained from one library consisted of Crenarchaeota and Euryarchaeota. These results demonstrate that molecular methods facilitate culture-independent studies, and that fish gut mucosa harbours a larger bacterial diversity than previously recognized. The grass carp intestinal habitat selects for specific bacterial taxa despite pronounced differences in host environments.