Mutating two putative phosphorylation sites on ZHP-3 does not affect its localization or function during meiotic chromosome segregation.
Mutating two putative phosphorylation sites on ZHP-3 does not affect its localization or function during meiotic chromosome segregation.
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DOI：
10.17912/micropub.biology.000354
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发表时间：
2021-01-18
影响因子：
--
通讯作者：
Bhalla N
中科院分区：
文献类型：
--
作者：
Russo AE;Nelson CR;Bhalla N
减数分裂染色体的分离依赖于交叉重组将同源染色体连接在一起并促进第一次减数分裂中的准确分离。在秀丽隐杆线虫中，一个保守的RING指蛋白ZHP-3是减数分裂重组所必需的，并定位于交换形成的位点。ZHP-3是否被调节以促进重组仍然知之甚少。体外分析鉴定了ZHP-3上两个推定的CHK-1激酶磷酸化位点。然而，体外鉴定的磷酸化位点的突变对ZHP-3的减数分裂重组或定位没有影响。因此，这两个磷酸化位点似乎与ZHP-3在减数分裂重组或其定位中的作用有关。
Meiotic chromosome segregation depends on crossover recombination to link homologous chromosomes together and promote accurate segregation in the first meiotic division. In Caenorhabditis elegans, a conserved RING finger protein, ZHP-3, is essential for meiotic recombination and localizes to sites of crossover formation. Whether ZHP-3 is regulated to promote recombination remains poorly understood. In vitro analysis identified two putative CHK-1 kinase phosphorylation sites on ZHP-3. However, mutation of the phosphorylation sites identified in vitro had no effect on meiotic recombination or localization of ZHP-3. Thus, these two phosphorylation sites appear to be dispensable for ZHP-3’s role in meiotic recombination or its localization.