Evaluation of bioaerosol samplers for the detection and quantification of influenza virus from artificial aerosols and influenza virus-infected ferrets
Evaluation of bioaerosol samplers for the detection and quantification of influenza virus from artificial aerosols and influenza virus-infected ferrets
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DOI：
10.1111/irv.12678
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发表时间：
2019-09-21
影响因子：
4.4
通讯作者：
Mubareka, Samira
中科院分区：
文献类型：
--
作者：
Bekking, Christian;Yip, Lily;Mubareka, Samira
背景生物气溶胶采样装置是表征由急性呼吸道病毒感染的自然感染宿主释放的感染性生物气溶胶所必需的。在多个实验条件下评估这些器械将为器械使用提供见解。目的本研究的主要目的是评估和比较生物气溶胶采样装置，使用a）在一系列不同RH条件下的体外环境受控人工生物气溶胶系统和B）在受控环境条件下感染流感病毒的雪貂的体内生物气溶胶系统。其次，我们还试图研究非甾体抗炎药对流感病毒感染的雪貂生物气溶胶排放的影响，以解决其作为生物气溶胶排放决定因素的潜力。方法采用人工生物气溶胶和雪貂流感病毒感染模型，对低、中容量生物气溶胶采样器在体外和体内采集病毒RNA和感染性流感病毒的性能进行了研究。测试了以下采样器：聚四氟乙烯过滤器（PTFE过滤器）、2级国家职业安全与健康研究所旋风采样器（NIOSH旋风采样器）和6级活Andersen撞击器（Andersen撞击器）。结果PTFE过滤器和NIOSH旋风采样器在一定相对湿度范围内从人工产生的气溶胶中收集到相似量的病毒RNA和感染性病毒。使用雪貂模型，PTFE过滤器、NIOSH旋风取样器和Andersen撞击器在峰回收率下分别收集高达3.66 log（10）拷贝的RNA/L空气、3.84 log（10）拷贝的RNA/L空气和6.09 log（10）拷贝的RNA/L空气。在病毒RNA回收的高峰日，从PTFE过滤器和NIOSH旋风取样器中回收感染性病毒。结论PTFE过滤器和NIOSH旋风采样器可用于流感病毒RNA和感染性病毒的采集，可考虑用于临床和环境设置。
Background Bioaerosol sampling devices are necessary for the characterization of infectious bioaerosols emitted by naturally-infected hosts with acute respiratory virus infections. Assessment of these devices under multiple experimental conditions will provide insight for device use. Objectives The primary objective of this study was to assess and compare bioaerosol sampling devices using a) an in vitro, environmentally-controlled artificial bioaerosol system at a range of different RH conditions and b) an in vivo bioaerosol system of influenza virus-infected ferrets under controlled environmental conditions. Secondarily, we also sought to examine the impact of NSAIDs on bioaerosol emission in influenza virus-infected ferrets to address its potential as a determinant of bioaerosol emission. Methods We examined the performance of low and moderate volume bioaerosol samplers for the collection of viral RNA and infectious influenza virus in vitroand in vivo using artificial bioaerosols and the ferret model of influenza virus infection. The following samplers were tested: the polytetrafluoroethylene filter (PTFE filter), the 2-stage National Institute of Occupational Safety and Health cyclone sampler (NIOSH cyclone sampler) and the 6-stage viable Andersen impactor (Andersen impactor). Results The PTFE filter and NIOSH cyclone sampler collected similar amounts of viral RNA and infectious virus from artificially-generated aerosols under a range of relative humidities (RH). Using the ferret model, the PTFE filter, NIOSH cyclone sampler and the Andersen impactor collected up to 3.66 log(10)copies of RNA/L air, 3.84 log(10)copies of RNA/L air and 6.09 log(10)copies of RNA/L air respectively at peak recovery. Infectious virus was recovered from the PTFE filter and NIOSH cyclone samplers on the peak day of viral RNA recovery. Conclusion The PTFE filter and NIOSH cyclone sampler are useful for influenza virus RNA and infectious virus collection and may be considered for clinical and environmental settings.