Treatment responses to surfactants containing natural surfactant proteins in preterm rabbits.
Treatment responses to surfactants containing natural surfactant proteins in preterm rabbits.
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早产兔对含有天然表面活性蛋白的表面活性剂的治疗反应。
DOI：
10.1164/ajrccm/147.3.669
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Jobe,AH
中科院分区：
文献类型：
--
作者：
Rider,ED;Ikegami,M;Whitsett,JA;Hull,W;Absolom,D;Jobe,AH
The in. vivofunction of surfactants reconstituted using natural surfactant lipid and protein constituents was evaluated in 27-day-gestation preterm rabbits. The animals were treated with protein-free surfactant lipids (LH-20), LH-20+ 5% SP-A, LH-20+ 1% SP-B, LH-20+ 1% SP-C, LH-20+ 5% SP-A+ 1% SP-B+ 1% SP-C (SP-ABC), natural sheep surfactant, or 4 mllkg 0.45% NaCI (control) and then ventilated with tidal volumes of 8 ml/kg and 3 cm H20 positive end-expiratory pressure (PEEP). Ventilatory pressures (peak pressures minus PEEP) and dynamic compliances of the LH-20+ SP-C rabbits were greater (p< 0.01) than those of control, LH-20, and LH-20+ SP-A groups but lower (p< 0.05) than those in the LH-20+ SP-B, LH-20+ SP-ABC, and sheep surfactant groups. Recoveries of intravascular labeled albumin in the lungs were comparable in the LH-20+ SP-B, LH-20+ SP-C, LH-20+ SP-ABC, and sheep surfactant groups and less (p< 0.01) than in LH-20+ SP-A rabbits, which had lower (p< 0.05) recoveries than did the control and LH-20 groups. The postventilation pressure-volume curves for LH-20+ SP-B and LH-20+ SP-ABC rabbits had significantly lower opening pressures, larger maximal lung volumes, and larger retained volumes on deflation relative to the LH-20+ SP-C, LH-20, and control groups. The effects of ventilation style on the responses were assessed by ventilating another group of similarly treated rabbits with tidal volumes of 10 mllkg and zero cm H20 PEEP. Without PEEp, only sheep surfactant demonstrated effective responses. In vivofunction of reconstituted surfactants containing SP-B was comparable to sheep surfactant, but, unlike natural surfactant, the activity was dependent upon a ventilation style using PEEP.Surfactant as recovered by alveolar lavage procedures is a complex mixture of phospholipids, neutrallipids, cholesterol, and at least three surfactant-associated proteins, SP-A, SP-B, and SP-C. The phospholipids are the components in the surfactant monolayer that reduce the surface tension at the alveolar airlliquid interphase. Full biologic activity of surfactant requires the interaction of the phospholipids with the associated protein components (1, 2). The specific role of each of the surfactant-associated proteins in promoting the activity of surfactant is currently not fully understood. SP-A, the major protein component of surfactant, is a glycosylated protein of 26 to 35 kD nominal monomeric molecular weight present in alveolar surfactant in a multimeric form of approximately 650 kD (2). The lipophilic low molecular weight proteins SP-B (5 to 18 kD, hydrophobic) and SP-C (3 to 6 kD, extremely hydrophobie) in surfactant extracts or phospholipid mixtures promote achievement of low surface tensions and rapid adsorption (3-9). SP-A acts synergistically with phospholipid mixtures containing the hydrophobic proteins to enhance biophysical perfor-
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与肺表面活性剂相关的人低分子量蛋白的分离和部分表征。
DOI：
--
发表时间：
1988
期刊：
American Review of Respiratory Disease
影响因子：
--
作者：
K. Kogishi;M. Kurozumi;Y. Fujita;T. Murayama;F. Kuze;Y. Suzuki
通讯作者：
Y. Suzuki
牛肺表面活性剂纯化低分子量疏水蛋白生物物理活性的比较研究。
DOI：
10.1016/0005-2760(88)90081-1
发表时间：
1988
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Yu Shou;Fred Possmayer
通讯作者：
Fred Possmayer
阻断相关蛋白 SP-A 和 SP-B 后肺表面活性剂的活性。
影响因子：
3.3
作者：
Tsutomu Kobayashi;Keiko Nitta;R. Takahashi;K. Kurashima;B. Robertson;Yasuhiro Suzuki
通讯作者：
Yasuhiro Suzuki
由产生 15 kDa 表面活性剂脱辅基蛋白抗体的杂交瘤引起的小鼠呼吸衰竭
影响因子：
2.1
作者：
Y. Suzuki;B. Robertson;Y. Fujita;G. Grossmann
通讯作者：
G. Grossmann
牛肺表面活性剂相关蛋白在磷脂混合物表面活性特性中的作用。
DOI：
10.1016/0005-2760(90)90236-q
发表时间：
1990
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Shou;Fred Possmayer
通讯作者：
Fred Possmayer