Triphenylamine pyridine acetonitrile fluorogens with green emission for pH sensing and application in cells
Triphenylamine pyridine acetonitrile fluorogens with green emission for pH sensing and application in cells
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绿色发射的三苯胺吡啶乙腈荧光剂用于 pH 传感及其在细胞中的应用
DOI：
10.1016/j.cclet.2014.11.029
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发表时间：
2015
影响因子：
9.1
通讯作者：
Zhou Xiang
中科院分区：
文献类型：
--
作者：
Wang Chang-Cheng;Yan Sheng-Yong;Chen Yu-Qi;Zhou Yi-Min;Zhong Cheng;Guo Pu;Huang Rong;Weng Xiao-Cheng;Zhou Xiang
本工作设计并合成了三苯胺吡啶乙腈类化合物--吡啶A1、吡啶A2和吡喃A3。它们在水中发出绿色荧光，在固体中发出明亮的光。它们有很大的行程移位(约100纳米)。研究了其在乙酸乙酯、氯仿、环己烷、乙醇、水、乙腈、四氢呋喃、DMF和DMSO中的激发光谱和发射光谱。有趣的是，这些化合物通过显示不同的颜色和发射强度，能够在pH 2到7的范围内感测到pH值的增加：在pH 2时几乎没有发射，在pH 7时有强烈的绿色发射。在紫外光照射下，在水溶液和1%琼脂糖凝胶中可以观察到酸碱转换的颜色和荧光的变化。这些变化可以重复几个周期。我们能够利用这些化合物进行细胞pH环境成像。
In this work, triphenylamine pyridine acetonitrile compoundsPyA1,PyA2andPyA3are designed and synthesized. They show green fluorescence in water and bright emission in solid state. They have large Strokes shift (about 100 nm). The excitation and emission spectra are investigated in ethyl acetate, chloroform, cyclohexane, ethanol, water, acetonitrile, THF, DMF and DMSO. It is interesting to find that these compounds are able to sense increasing pH values ranging from pH 2 to 7 by showing different color and emission intensities: almost no emission at pH 2 and strong green emission at pH 7. The acid–base switched color and fluorescence changes can be observed under UV irradiation, in water solution and 1% agarose gel. These changes can be repeated for several cycles. We are able to employ these compounds for cell pH environment imaging.