Weak magnetic fields modulate superoxide to control planarian regeneration
Weak magnetic fields modulate superoxide to control planarian regeneration
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DOI：
10.3389/fphy.2022.1086809
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发表时间：
2023-01-04
影响因子：
3.1
通讯作者：
Beane, Wendy S.
中科院分区：
文献类型：
--
作者：
Kinsey, Luke J.;Van Huizen, Alanna V.;Beane, Wendy S.
活性氧（ROS）信号调节细胞行为和组织生长的发展，再生和癌症。通常，ROS是受调节的，其虽然有效，但伴随着潜在的并发症，如脱靶效应和缺乏药物耐受性。因此，需要额外的非侵入性治疗方法。最近的进展强调了弱磁场（WMF，< 1 mT）的使用是一种有前途的方法。我们以前表明，200亩T WMF抑制ROS的形成和阻断真涡虫再生。然而，在不同场强下对不同模型系统的WMF研究产生了一系列不符合常见剂量反应曲线的结果，因此不清楚WMF效应是否可预测。在这里，我们测试假设的基础上自旋状态理论和自由基对机制，它概述了磁场如何可以改变形成的自由基对通过改变电子自旋状态。这种机制表明，在广泛的场强范围（0-900 μ T），一些WMF曝光应该能够抑制，而其他促进ROS形成的二元方式。我们的数据表明，WMF可用于以可预测的方式直接操纵干细胞增殖、分化和组织生长，以在再生生长期间丧失和获得功能。此外，我们检查了两种最常见的活性氧信号效应物，过氧化氢和超氧化物，以开始识别和阐明WMF影响组织生长的特定分子靶点。总之，我们的数据表明，WMF暴露的细胞效应高度依赖于ROS，我们确定超氧化物作为一个特定的ROS被调制。总之，这些数据突出了使用WMF暴露来控制体内ROS信号传导的可能性，并代表了一个令人兴奋的新研究领域。
Reactive oxygen species (ROS) signaling regulates cell behaviors and tissue growth in development, regeneration, and cancer. Commonly, ROS are modulated pharmacologically, which while effective comes with potential complications such as off-target effects and lack of drug tolerance. Thus, additional non-invasive therapeutic methods are necessary. Recent advances have highlighted the use of weak magnetic fields (WMFs, < 1 mT) as one promising approach. We previously showed that 200 mu T WMFs inhibit ROS formation and block planarian regeneration. However, WMF research in different model systems at various field strengths have produced a range of results that do not fit common dose response curves, making it unclear if WMF effects are predictable. Here, we test hypotheses based on spin state theory and the radical pair mechanism, which outlines how magnetic fields can alter the formation of radical pairs by changing electron spin states. This mechanism suggests that across a broad range of field strengths (0-900 mu T) some WMF exposures should be able to inhibit while others promote ROS formation in a binary fashion. Our data reveal that WMFs can be used for directed manipulation of stem cell proliferation, differentiation, and tissue growth in predictable ways for both loss and gain of function during regenerative growth. Furthermore, we examine two of the most common ROS signaling effectors, hydrogen peroxide and superoxide, to begin the identification and elucidation of the specific molecular targets by which WMFs affect tissue growth. Together, our data reveal that the cellular effects of WMF exposure are highly dependent on ROS, and we identify superoxide as a specific ROS being modulated. Altogether, these data highlight the possibilities of using WMF exposures to control ROS signaling in vivo and represent an exciting new area of research.